文献类型：期刊文献

英文题名：A peptide-based conductive hydrogel capable of in situ bioelectrocatalytic NADH regeneration for sustained production of 1-propanol

作者：Liu, Yaping[1]; Lv, Mingchen[1]; Chen, Ran[1]; Zhang, Zhuo[1]; Teng, Runxin[1]; Qin, Jinlong[2]; Sun, Min[2]; Fan, Zhen[1,2]; Du, Jianzhong[1,2,3]

机构：[1] School of Materials Science and Engineering, Tongji University, Shanghai, 201804, China; [2] Department of Gynaecology and Obstetrics, Shanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Clinical Research Center for Anesthesiology and Perioperative Medicine, Translational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai, 200434, China; [3] School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China

年份：2024

卷号：13

期号：4

起止页码：2780

外文期刊名：Journal of Materials Chemistry A

收录：EI(收录号：20245117565472)

语种：英文

外文关键词：Coenzymes

摘要：Nicotinamide-adenine dinucleotide (NAD+/NADH) is a coenzyme that acts as a donor and acceptor of electrons in redox reactions within all eukaryotic cells. The reduction of NAD+ produces NADH that subsequently acts as the key electron carrier. However, limited stability of enzymes under operational conditions, unwanted by-products and limited external electron donors result in low efficiency of NADH regeneration to drive the downstream enzymatic reactions. Herein, we modularly designed a peptide hydrogel, which provided an appropriate physiological microenvironment for maintaining the bioactivity of alcohol dehydrogenase. Meanwhile, Bi nanoparticles were in situ synthesized and doped into nanofibers during the peptide self-assembly to afford a conductive hydrogel with enhanced bioelectrocatalytic efficiency. Both linear sweep voltammetry and chronoamperometry data unveiled excellent electrochemical performance of this hydrogel for sustained NADH regeneration at low potential (?0.37 V vs. RHE). Even at a relatively high potential of ?0.79 V vs. RHE, the yield of NADH for 10 h was as high as 81%. The regenerated NADH could further drive propionaldehyde alcoholization to produce 1-propanol sustainably with a rate of 1.34 mM h?1 at ?0.62 V (vs. RHE). Overall, this peptide-based conductive hydrogel could achieve sustained 1-propanol production by leveraging in situ bioelectrocatalytic NADH regeneration, demonstrating a promising approach for electroenzymatic biofuel and pharmaceutical production. ? The Royal Society of Chemistry 2025.