文献类型：期刊文献

英文题名：Resource utilization of penicillin fermentation fungi residue for ectoine production by Halomonas elongata

作者：Liu, Yuxuan[1];Zhang, Sinan[1];Zhang, Guohui[1];Zhang, Yue[2];Yang, Chen[2];Wang, Zejian[2];Yu, Junxiong[2];Wu, Shiyong[1,3]

机构：[1]East China Univ Sci & Technol, Dept Chem Engn Energy Resources, Natl Key Lab Coal Liquificat Gasificat & Utilizat, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Dept Biotechnol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[3]East China Univ Sci & Technol, Engn Res Ctr Resource Utilizat Carbon Containing W, Minist Educ, Shanghai 200237, Peoples R China

年份：2025

卷号：436

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20252918819736);WOS:【SCI-EXPANDED(收录号:WOS:001540048100001)】；

基金：This work was supported by the financial support of research projects from the National Key R & D Program of China (2020YFA0906800) , High-quality development project CEIEC-2022-ZM02-0259, Shanghai Scientific and Technological Innovation Action Plans-Scientific In-strument Development, China (grant No. 21142201300, 22142201000) , National Natural Science Foundation of China (No. 32071471) .

语种：英文

外文关键词：PFFR; Hydrolysate; Ectoine; Halomonas elongata; Resource utilization

摘要：Penicillin fermentation fungal residue (PFFR), a hazardous waste with over 90 % organic matter, can pollute the environment if not properly disposed of. In this study, acid/alkaline heat pretreatment was employed to remove antibiotic residues and break down macromolecular compounds into smaller molecules. The hydrolysate contained 17.23 g/L of total amino acids, 5.34 g/L of reducing sugars, and 33.6 g/L of salts. PFFR hydrolysate was then used as the base medium for the halophile Halomonas elongata to produce the high-value compound ectoine. Response surface methodology was used to optimize medium conditions, resulting in a maximum ectoine concentration of 3957.06 +/- 214.12 mg/L. Additionally, a cost-effective mixture of corn steep liquor and hydrolysate was employed for fed-batch cultivation, combined with gradient salt shock to optimize fermentation. The final ectoine yield reached 15837.02 +/- 422.27 mg/L. This study offers a cost-effective, environmentally friendly, and efficient strategy for the resource utilization of PFFR.