文献类型：期刊文献

英文题名：Identification of BagI as a positive transcriptional regulator of bagremycin biosynthesis in engineered Streptomyces sp Tu 4128

作者：Liu, Feng[1];Xu, Dakui[1];Zhang, Yuchen[1];Zhu, Yunxia[1];Ye, Jiang[1];Zhang, Huizhan[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：173

起止页码：18

外文期刊名：MICROBIOLOGICAL RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000353080200003)】；

基金：This work was supported by a grant from National Natural Science Foundation of China (C010201). We are particularly thankful to Prof. Hans-Peter Fiedler for providing strain S. sp. Tu 4128 and bagremycins samples. We also thank Prof. Changsheng Zhang for sharing strain E. coli ET12567/pUZ8002 and plasmid pSET152 with us.

语种：英文

外文关键词：Bagremycin; bagI; Streptomyces sp Tu 4128; Morphological differentiation; Transcriptional regulation

摘要：Bagremycin A and B, two novel antibiotics from Streptomyces sp. Tu 4128, show a moderate activity against fungi, Gram-positive bacteria and tumor cell and have potential application values in the fields of medicine and agriculture. In this study, we obtained a bagI deletion mutant by in-frame deletion. The assays of bagI-deletion and complementation strains revealed that it was essential to bagremycin biosynthesis. Secondly, the impact of bagI mutants on mycelial growth, sporulation and pigment yields was explored throughout secondary metabolism. SEM images displayed that bagI mutation delayed the mycelium growth and sporulation and reduced pigment yields. Moreover, the yields of bagremycin A and B increased 2.5-fold and 2.6-fold in bagI-overexpressed strain compared to WT. Thirdly, we investigated genes associated with bagremycin biosynthesis by real-time fluorescent quantitative PCR (qRT-PCR). Data showed that BagI played a role of transcriptional activator in the course of bagremycin biosynthesis. This provides us new insights into the regulatory network of bagremycin biosynthesis. (C) 2015 Elsevier GmbH. All rights reserved.