文献类型：期刊文献

英文题名：One-Pot Enzyme Cascade for Controlled Synthesis of Furancarboxylic Acids from 5-Hydroxymethylfurfural by H2O2 Internal Recycling

作者：Jia, Hao-Yu[1];Zong, Min-Hua[1];Zheng, Gao-Wei[2];Li, Ning[1]

机构：[1]South China Univ Technol, Sch Food Sci & Engn, State Key Lab Pulp & Paper Engn, 381 Wushan Rd, Guangzhou 510640, Guangdong, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2019

卷号：12

期号：21

起止页码：4764

外文期刊名：CHEMSUSCHEM

收录：;EI(收录号：20194407596278);WOS:【SCI-EXPANDED(收录号:WOS:000495128100004)】；

语种：英文

外文关键词：biocatalysis; biomass; dehydrogenases; heterocycles; oxidases

摘要：Furancarboxylic acids are promising biobased building blocks in pharmaceutical and polymer industries. In this work, dual-enzyme cascade systems composed of galactose oxidase (GOase) and alcohol dehydrogenases (ADHs) are constructed for controlled synthesis of 5-formyl-2-furancarboxylic acid (FFCA) and 2,5-furandicarboxylic acid (FDCA) from 5-hydroxymethylfurfural (HMF), based on the catalytic promiscuity of ADHs. The byproduct H2O2, which is produced in GOase-catalyzed oxidation of HMF to 2,5-diformylfuran (DFF), is used for horseradish peroxidase (HRP)-mediated regeneration of the oxidized nicotinamide cofactors for subsequent oxidation of DFF promoted by an ADH, thus implementing H2O2 internal recycling. The desired products FFCA and FDCA are obtained with yields of more than 95 %.