文献类型：期刊文献

英文题名：Antioxidative cellular response of lepidopteran ovarian cells to photoactivated alpha-terthienyl

作者：Huang, Qingchun[1];Yun, Xinming[1];Rao, Wenbing[1];Xiao, Ciying[2]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2017

卷号：137

起止页码：1

外文期刊名：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000399067000001)】；

基金：This work was financially supported by Shanghai Foundation of Science and Technology (No. 15431906800). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：Alpha-terthienyl; Tn5B1-4 cells; Sf-21 cells; Antioxidative response; Cytotoxicity

摘要：Photodynamic sensitizers as useful alternative agents have been used for population control against insect pests, and the response of insect ovarian cells towards the photosensitizers is gaining attention because of the next reproduction. In this paper, antioxidative responses of lepidopteran ovarian Tn5B1-4 and Sf-21 cells to photoactivated alpha-terthienyl (PAT) are investigated. PAT shows positive inhibitory cytotoxicity on the two ovarian cells, and its inhibition on cell viability is enhanced as the concentrations are increased and the irradiation time is extended. Median inhibitory concentrations (IC50) are 336 mu g/ml to Tn5B1-4 cells, and 3.15 mu g/ml to Sf-21 cells at 15 min-UV-A irradiation 2 h-dark incubation. Under 10.0 mu g/ml PAT exposure, 15 min-UV-A irradiation excites higher ROS production than 5 min-UV-A irradiation does in the ovarian cells, the maximum ROS content is about 7.1 times in Tn5B1-4 cells and 4.3 times in Sf-21 cells, and the maximum malondialdehyde levels in Tn5B1-4 and Sf-21 cells are about 1.47- and 1.36-fold higher than the control groups, respectively. Oxidative stress generated by PAT strongly decreases the activities of POD, SOD and CAT, and induces an accumulation of Tn5B1-4 cells in S phase and Sf-21 cells in G2/M phase in a concentration-dependent fashion. Apoptosis accumulation of Tn5B1-4 cells and the persistent post-irradiation cytotoxicity are further observed, indicating different antioxidative tolerance and arrest pattern of the two ovarian cells towards the cytotoxicity of PAT. (C) 2016 Elsevier Inc. All rights reserved.