文献类型：期刊文献

英文题名：A novel strain Pseudomonas aeruginosa CS-2 producing an organic solvent-tolerant lipase

作者：Peng, Ren[1,2]; Lin, Jinping[1]; Wei, Dongzhi[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; [2] College of Life Science, Jiangxi Normal University, Nanchang 330022, China

年份：2012

卷号：343-344

起止页码：774

外文期刊名：Advanced Materials Research

收录：EI(收录号：20114114417984)

语种：英文

外文关键词：Bacteria - Lipases - Hexane - Magnesium compounds - Organic solvents - Chlorine compounds

摘要：Organic solvent-tolerant lipases have shown new potential application in biotechnological fields. A novel strain which produced an organic solvent-tolerant lipase was isolated from soils in different areas of China. Based on the morphological, physiological and biochemical features and 16S rDNA sequence of the strain, it was identified as Pseudomonas aeruginosa CS-2. The crude lipase had an improved stability in the presence of acetonitrile and it exhibited stability in presence of benzene, chloroform, n-Hexane, petroleum ether and isooctane. The optimized composition of culture medium was as follows: olive oil 1%, peptone 4%, K2HPO4 0.5%, MgSO 4·7H2O 0.1%, gum arabic 0.04%. The optimum condition for lipase production was on the following: temperature 37°C, initial pH 7.5, rotary shaking speed 200 rev/min, culture time 48 h. ? (2012) Trans Tech Publications, Switzerland.