文献类型：期刊文献

英文题名：Bioaugmentation of intertidal sludge enhancing the development of salt-tolerant aerobic granular sludge

作者：Yao, Jin-chi[1,2];Yao, Gen-ji[1,2];Wang, Zu-hao[1,2];Yana, Xin-jie[1,2];Lu, Qing-qing[1,2];Li, Wei[1,2,3];Liu, Yong-di[1,2,3]

机构：[1]East China Univ Sci & Technol, Natl Engn Res Ctr Ind Wastewater Detoxicat & Resou, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Sch Resources & Environm Engn, State Environm Protect Key Lab Environm Risk Asses, Shanghai, Peoples R China;[3]Shanghai Inst Pollut Control & Ecol Secur, Shanghai, Peoples R China

年份：2023

卷号：325

外文期刊名：JOURNAL OF ENVIRONMENTAL MANAGEMENT

收录：;EI(收录号：20233214523406);WOS:【SCI-EXPANDED(收录号:WOS:000889612700001)】；

基金：This work was supported by the National Key Research and Development Program of China (2019YFC0408202), the National Natural Science Foundation of China (52170076), the Shanghai Rising-Star Program (20QC1400500), and the State Key Laboratory of Pollution Control and Resource Reuse (PCRRF20024).

语种：英文

外文关键词：Intertidal sludge; Salt-tolerant aerobic granule sludge; Rapid development; Salt-tolerant potential; Metagenomics

摘要：Three parallel bioreactors were operated with different inoculation of activated sludge (R1), intertidal sludge (Its) (R2), and ItS-added AS (R3), respectively, to explore the effects of ItS bioaugmentation on the formation of salt-tolerant aerobic granular sludge (SAGS) and the enhancement of COD removal performance. The results showed that compared to the control (R1-2), R3 promoted a more rapid development of SAGS with a cultivation time of 25 d. Following 110-day cultivation, R3 exhibited a higher granular diameter of 1.3 mm and a higher hydrophobic aromatic protein content than that in control. Compared to the control, the salt-tolerant performance in R3 was also enhanced with the COD removal efficiency of 96.4% due to the higher sludge specific activity of 14.4 g.gVSS(-1).d(-1) and the salinity inhibition constant of 49.3 gL(-1). Read- and genome-resolved metagenomics together indicated that a higher level of tryptophan/tyrosine synthase gene (trpBD, tyrBC) and enrichment of the key gene hosts Rhodobacteraceae, Marinicella in R3, which was about 5.4-fold and 1.4-fold of that in control, could be the driving factors of rapid development of SAGS. Furthermore, the augmented salt-tolerant potential in R3 could result from that R1 was dominated by Rhodospirillaceae, Bacteroidales, which carried more trehalose synthase gene (otsB, treS), while the dominant members Rhodobacteraceae, Marinicella in R3 were main contributors to the glycine betaine synthase gene (ectC, betB, gbsA). This study could provide deeper insights into the rapid development and improved salt-tolerant potential of SAGS via bioaugmentation of intertidal sludge, which could promote the application of hypersaline wastewater treatment.