文献类型：期刊文献

英文题名：Improved production of erythromycin a by expression of a heterologous gene encoding S-adenosylmethionine synthetase

作者：Wang, Yong[1,2]; Wang, YiGuang[1,2]; Chu, Ju[1]; Zhuang, Yingping[1]; Zhang, Lixin[3,4,5,6]; Zhang, Siliang[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; [2] Institute of Medical Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Tiantan, Beijing 100050, China; [3] Institute of Microbiology, Chinese Academy of Sciences [CAS], Beijing 100080, China; [4] South China Sea Institute of Oceanology, Chinese Academy of Sciences [CAS], Beijing 510301, China; [5] Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences [CAS], Guangzhou 510663, China; [6] SynerZ Pharmaceuticals, Lexington, MA 02421, United States

年份：2007

卷号：75

期号：4

起止页码：837

外文期刊名：Applied Microbiology and Biotechnology

收录：EI(收录号：20073110739922)

语种：英文

外文关键词：Bioassay - Chromosomes - Enzymes - Gene encoding - High performance liquid chromatography

摘要：An S-adenosylmethionine synthetase (SAM-s) gene from Streptomyces spectabilis was integrated along with vector DNA into the chromosome of a Saccharopolyspora erythraea E2. Elevated production of SAM was observed in the recombinant strain Saccharopolyspora erythraea E1. The results from the bioassay showed that the titer of erythromycin was increased from 920 IU ml-1 by E2 to approximately 2,000 IU ml-1 by E1. High performance liquid chromatography (HPLC) analysis revealed that there was a 132% increase in erythromycin A compared with the original strain, while the erythromycin B, the main impurity component in erythromycin, was decreased by 30%. The sporulation process was inhibited, while the SAM-s gene was expressed. The addition of the exogenous SAM also inhibited sporulation and promoted an increase in erythromycin titers. ? 2007 Springer-Verlag.