文献类型：期刊文献

英文题名：Statistic Copolymers Working as Growth Factor-Binding Mimics of Fibronectin

作者：Zhang, Wenjing[1];Wu, Yueming[2];Chen, Qi[2];Zhang, Haodong[2];Zhou, Min[1];Chen, Kang[2];Cao, Chuntao[2];Guo, Han[3];Xu, Jianrong[4];Liu, Honglai[5];Lin, Haodong[6];Liu, Changsheng[2];Liu, Runhui[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Sch Mat Sci & Engn,Res Ctr Biomed Mat,Frontiers S, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai Synchrotron Radiat Facil SSRF, Shanghai 201204, Peoples R China;[4]Shanghai Univ Tradit Chinese Med, Acad Integrat Med, Shanghai 201203, Peoples R China;[5]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[6]Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Sch Med, Dept Orthoped Surg, Shanghai 200080, Peoples R China

年份：2022

卷号：9

期号：21

外文期刊名：ADVANCED SCIENCE

收录：;EI(收录号：20222012126380);WOS:【SCI-EXPANDED(收录号:WOS:000795737300001)】；

基金：This research was supported by the National Natural Science Foundation of China for Innovative Research Groups (No. 51621002), the National Natural Science Foundation of China (No. 31800801), Program of Shanghai Academic/Technology Research Leader (20XD1421400), Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission, grant 2021 Sci & Tech 03-28), Research program of State Key Laboratory of Bioreactor Engineering, the Fundamental Research Funds for the Central Universities (JKD01211520). The authors also thank Research Center of Analysis and Test of East China University of Science and Technology for the help on the characterization.

语种：英文

外文关键词：fibronectin mimicking; growth factor binding; statistic copolymers

摘要：Growth factors (GFs) play important roles in biological system and are widely used in tissue regeneration. However, their application is greatly hindered by short in vivo lifetime of GFs. GFs are bound to fibronectin dynamically in the extracellular matrix, which inspired the authors to mimic the GF binding domain of fibronectin and design GF-binding amphiphilic copolymers bearing positive charges. The optimal amino acid polymer can bind to a variety of representative GFs, such as bone morphogenetic protein-2 (BMP-2) and TGF-beta 1 from the transforming growth factor-beta superfamily, PDGF-AA and PDGF-BB from the platelet-derived growth factor family, FGF-10 and FGF-21 from the fibroblast growth factor family, epidermal growth factor from the EGF family and hepatocyte growth factor from the plasminogen-related growth factor family, with binding affinities up to the nanomolar level. 3D scaffolds immobilized with the optimal copolymer enable sustained release of loaded BMP-2 without burst release and significantly enhances the in vivo function of BMP-2 for bone formation. This strategy opens new avenues in designing GF-binding copolymers as synthetic mimics of fibronectin for diverse applications.