文献类型：期刊文献

英文题名：High-Throughput Screening for a Moderately Halophilic Phenol-Degrading Strain and Its Salt Tolerance Response

作者：Lu, Zhi-Yan[1];Guo, Xiao-Jue[1];Li, Hui[1];Huang, Zhong-Zi[1,2];Lin, Kuang-Fei[1];Liu, Yong-Di[1]

机构：[1]E China Univ Sci & Technol, Sch Resources & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China;[2]Shanghai Univ Engn Sci, Sch Chem Engn, Shanghai 201620, Peoples R China

年份：2015

卷号：16

期号：6

起止页码：11834

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000357492800001)】；

基金：Supported jointly by the National Natural Science Foundation of China (51378208, 41273109, 41003031), the Specialized Research Fund for the Doctoral Program of Higher Education (20110074130002), the Program for New Century Excellent Talents in University (NCET-13-0797), the Fok Ying Tung Education Foundation (141077), the Innovation Program of Shanghai Municipal Education Commission (14ZZ059) and the Fundamental Research Funds for the Central Universities (222201313008). We also would like to thank the anonymous referees for their helpful comments on this paper.

语种：英文

摘要：A high-throughput screening system for moderately halophilic phenol-degrading bacteria from various habitats was developed to replace the conventional strain screening owing to its high efficiency. Bacterial enrichments were cultivated in 48 deep well microplates instead of shake flasks or tubes. Measurement of phenol concentrations was performed in 96-well microplates instead of using the conventional spectrophotometric method or high-performance liquid chromatography (HPLC). The high-throughput screening system was used to cultivate forty-three bacterial enrichments and gained a halophilic bacterial community E3 with the best phenol-degrading capability. Halomonas sp. strain 4-5 was isolated from the E3 community. Strain 4-5 was able to degrade more than 94% of the phenol (500 mg center dot L-1 starting concentration) over a range of 3%-10% NaCl. Additionally, the strain accumulated the compatible solute, ectoine, with increasing salt concentrations. PCR detection of the functional genes suggested that the largest subunit of multicomponent phenol hydroxylase (LmPH) and catechol 1,2-dioxygenase (C12O) were active in the phenol degradation process.