文献类型：期刊文献

英文题名：Succinic acid production and CO₂ fixation using a metabolically engineered Escherichia coli in a bioreactor equipped with a self-inducing agitator

作者：Wu, Hui[1];Li, Qing[1];Li, Zhi-min[1];Ye, Qin[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2012

卷号：107

起止页码：376

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000301620600053)】；

基金：The authors thank Professor David P. Clark (Southern Illinois University, USA) for kindly providing us with the strain, E. coli NZN111, and Joanna Jan and Mary Harrison (Rice University, USA) for read-through of this manuscript. This work was supported by the National Natural Science Foundation of China (Grant No. 20576032), the National High Technology Research and Development Program of China (Grant No. 2011AA02A203), and the National Special Fund for State Key Laboratory of Bioreactor Engineering, Grant No. 2060204.

语种：英文

外文关键词：Escherichia coli; Succinic acid; CO2 fixation; Self-induction agitator; Bioreactor

摘要：A 5-L bioreactor equipped with a self-induction agitator was applied to a two-stage culture of Escherichia coli NZN111 for succinic acid production in a mineral salts medium. CO2 was cycled inside this reactor and a sufficient CO2 transfer rate was maintained with the elimination of CO2 wasted by ventilation. In the anaerobic stage, much less supplemental CO2 was required at pH 6.3 compared to that at pH 7.0, and the succinate yield increased. The performances of succinate production were little changed when compared to a process with CO2 sparging indicating that use of the self-inducing agitator reduced CO2 waste. The succinate production process was further coupled with ethanol fermentation by using the CO2 produced from ethanol fermentation. This integrated system demonstrated that both succinate and bioethanol can be effectively produced while the emission of the CO2 formed during ethanol fermentation can be greatly reduced. (C) 2011 Elsevier Ltd. All rights reserved.