文献类型：期刊文献

中文题名：葡萄糖氧化酶发酵动力学的研究

英文题名：Kinetics of Microbial Growth and Glucose Oxidase Formation

作者：储炬[1];李友荣[1];俞俊棠[1]

机构：[1]华东理工大学生化工程系

年份：1997

卷号：23

期号：3

起止页码：303

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：1997123980610);Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金

语种：中文

中文关键词：葡萄糖氧化酶;发酵动力学;恒化培养;酶;比生长速率

外文关键词：glucose oxidase; kinetics; chemostat culture; enzyme; specific growth rate

摘要：利用恒化器研究了葡萄糖氧化酶（ＧＯＤ）发酵动力学，建立了较为合理的恒化培养系统。对采集的数据用计算机进行回归，求得最大比生长速率μｍａｘ＝０．２２５ｈ－１、饱和常数Ｋｓ＝８．９１ｇ／Ｌ、基质得率系数ＹＧｍａｘ＝０．４９ｇ／ｇ以及维持系数ｍ＝０．０２ｇ／ｇ·ｈ。并研究了ｐＨ、ＤＯ、ＮＨ２－Ｎ、葡萄糖酸、细胞产率，酶比活及体积产酶速率等参数随比生长速率变化的规律。在Ｄ＝０．１３１ｈ－１下体积产酶速率（ＤＥ）达最大值，为３．４６×１６．６７μｍｏｌ／ｓ·Ｌ·ｈ，是分批发酵最大体积产酶速率（１．５３×１６．６７μｍｏｌ／ｓ·Ｌ·ｈ）的２．２６倍。
The relationship between various parameters and the dilution rate were investigated in chemostat culture system. The results of which show that the maximum specific growth rate, μ max =0.225h -1 ,substrate constant, K S=8.91g/L,yield of the substrate, Y g max =0.49g/g and maintenance coefficient, m =0.02g/g·h. The relationship between specific growth rate and DO, NH 2 N, gluconic acid, biomass productivity, specific enzyme activity, enzyme volumetric productivity were studied. The results of continuous culture show that when glucose was served as limited substrate and D =0.131h -1 , the maximum value of volumetric enzyme productivity ( D·E) ,3.46×16.67μmol/(s·L·h) was obtained, which was 2.2 times of the maximum volumetric enzyme productivity, 1.53×16.67μmol/(s·L·h), of batch fermentation.