文献类型：期刊文献

英文题名：Genome-based mining of new antimicrobial meroterpenoids from the phytopathogenic fungus Bipolaris sorokiniana strain 11134

作者：Han, Jianying[1,2];Zhang, Jingyu[3];Song, Zhijun[2];Zhu, Guoliang[3];Liu, Miaomiao[1];Dai, Huanqin[2];Hsiang, Tom[4];Liu, Xueting[3];Zhang, Lixin[2,3];Quinn, Ronald J.[1];Feng, Yunjiang[1]

机构：[1]Griffith Univ, Griffith Inst Drug Discovery, Brisbane, Qld 4111, Australia;[2]Chinese Acad Sci, Inst Microbiol, Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]Univ Guelph, Sch Environm Sci, Guelph, ON N1G 2W1, Canada

年份：2020

卷号：104

期号：9

起止页码：3835

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20201508403109);WOS:【SCI-EXPANDED(收录号:WOS:000521784500002)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (81573341, 31720103901, and 21877038). This work was partially supported by the Open Project Funding of the State Key Laboratory of Bioreactor Engineering, the 111 Project (B18022), the Fundamental Research Funds for the Central Universities (22221818014), and Taishan Scholarship. This work was also supported by the Australian Research Council (ARC) (DP160101429, LE140100119, and LE120100170). The genome sequencing and assembly of BS11134 was supported by funding from the Natural Science and Engineering Research Council of Canada to T. Hsiang.

语种：英文

外文关键词：Meroterpenoids; Phytopathogenic fungus; Bipolaris sorokiniana; Genome-based mining; Antimicrobial activity

摘要：Polyketide-terpenoid hybrid compounds are one of the largest families of meroterpenoids, with great potential for drug development for resistant pathogens. Genome sequence analysis of secondary metabolite gene clusters of a phytopathogenic fungus, Bipolaris sorokiniana 11134, revealed a type I polyketide gene cluster, consisting of highly reducing polyketide synthase, non-reducing polyketide synthase, and adjacent prenyltransferase. MS- and UV-guided isolations led to the isolation of ten meroterpenoids, including two new compounds: 19-dehydroxyl-3-epi-arthripenoid A (1) and 12-keto-cochlioquinone A (2). The structures of 1-10 were elucidated by the analysis of NMR and high-resolution electrospray ionization mass spectroscopy data. Compounds 5-8 and 10 showed moderate activity against common Staphylococcus aureus and methicillin-resistant S. aureus, with minimum inhibitory concentration (MIC) values of 12.5-100 mu g/mL. Compound 5 also exhibited activity against four clinical resistant S. aureus strains and synergistic antifungal activity against Candida albicans with MIC values of 12.5-25 mu g/mL. The biosynthetic gene cluster of the isolated compounds and their putative biosynthetic pathway are also proposed.