文献类型：期刊文献

英文题名：Absorption Characteristics of Chitobiose and Chitopentaose in the Human Intestinal Cell Line Caco-2 and Everted Gut Sacs

作者：Chen, Ping[1];Zhao, Mengyao[1];Chen, Qiming[1];Fang, Liqiang[1];Gao, Feng[2];Zhao, Liming[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, R&D Ctr Separat & Extract Technol Fermentat Ind, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[3]SCICBT, Shanghai 200237, Peoples R China

年份：2019

卷号：67

期号：16

起止页码：4513

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20191806853117);WOS:【SCI-EXPANDED(收录号:WOS:000466052700013)】；

基金：This work was supported by the National Natural Science Foundation of China (no. 31371725), The National Key Research and Development Program of China (2017YFB0309302), "Shu Guang" project of Shanghai Municipal Education Commission and Shanghai Education Development Foundation (15SG28), Fundamental Research Funds for the Central Universities (22221818014), and partially supported by the Open Project Funding of the State Key Laboratory of Bioreactor Engineering, ECUST (ZDXM2019).

语种：英文

外文关键词：chitooligosaccharide; everted gut sac model; intestinal absorption; Caco-2 cell model; glucose transporter 2 (GLUT2)

摘要：The everted gut sacs and Caco-2 cell models were used to investigate the intestinal absorptive characteristics and subcellular localization of chitobiose and chitopentaose in this study. In everted gut sacs, the absorptive pattern showed no concentration-dependent manner when the concentration was lower than 10 mM. In the presence of phlorizin (100 mu M) and phloretin (100 mu M), the chitobiose absorption rates decreased by (4.97 +/- 0.89)% and (19.2 +/- 2.77)%, and they were (10.4 +/- 2.43)% and (27.5 +/- 1.68)% for chitopentaose. In Caco-2 cells, the concentration showed influences similar to those with the everted gut sacs results. After adding phlorizin and phloretin in the apical side, the PappAP-BL of chitobiose and chitopentaose significantly decreased. Considering the translocation, they were enriched in endoplasmic reticulum and mitochondrion. This study indicated that concentration and active transporter were capable of mediating the absorption of chitobiose and chitopentaose, and the subcellular localization of them could help to study the mechanisms of their effects.