文献类型：专利

英文题名：Horse radish peroxidase-oxidase in -3 -acetic acid reaction pH change detection method, involves adding pH sensitive ratio of fluorescent protein pHluorins in horseradish peroxide enzyme-indol -3 -acetic acid reaction system

作者：LIU H;WANG P;CAO X

机构：[1]UNIV EAST CHINA SCI & TECHNOLOGY

申请号：CN103630519-A

申请日：2013-12-03

公开日：2014-03-12

语种：英文

收录：DERWENT

摘要：NOVELTY - The method involves adding pH sensitive ratio of fluorescent protein pHluorins in horseradish peroxide enzyme-indol -3 -acetic acid reaction system. Fluorescent intensity is changed according to ratio of fluorescent protein pHluorins of pH-sensitive to determine of the pH change in reaction process. A fluorescence intensity value of an excitation wavelength is ranged as 397nm/nm emission wavelength of 507nm and measured. A reaction system is provided with a reaction medium i.e. water or buffer solution. USE - Horse radish peroxidase-oxidase in -3 -acetic acid reaction pH change detection method. ADVANTAGE - The method utilizes pH-sensitive ratio of fluorescent protein to reflect the horseradish peroxidase enzyme (HRP) and the prodrug of pH change in -3 -acetic acid (IAA) of reaction process, and simplifies fluorescence enzyme labeling instrument to obtain pH change result of real-time monitoring of reaction. DESCRIPTION OF DRAWING(S) - The drawing shows a graphical representation illustrating Horse radish peroxidase-oxidase in -3 -acetic acid reaction pH change detection method.