文献类型：期刊文献

英文题名：Visualization of Nicotine Adenine Dinucleotide Redox Homeostasis with Genetically Encoded Fluorescent Sensors

作者：Zhao, Yuzheng[1,2];Zhang, Zhuo[1,2];Zou, Yejun[1,2];Yang, Yi[1,3]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg Technol, State Key Lab Bioreactor Engn, Synthet Biol & Biotechnol Lab, 130 Mei Long Rd, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, 130 Mei Long Rd, Shanghai, Peoples R China;[3]Chinese Acad Sci, Shanghai Inst Biol Sci, CAS Ctr Excellence Brain Sci, Optogenet & Synthet Biol Interdisciplinary Res Ct, Shanghai, Peoples R China

年份：2018

卷号：28

期号：3

起止页码：213

外文期刊名：ANTIOXIDANTS & REDOX SIGNALING

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000418408800004)】；

基金：This research was supported by the National Key Research and Development Program of China (2013CB531200 and 2017YFA0504000 to Y.Y., SQ2017YFSF090219 to Y.Z.), NSFC (91313301, 31225008, and 31470833 to Y.Y., 91649123 and 31671484 to Y.Z.), the Shanghai Science and Technology Commission (14XD1401400 and 16430723100 to Y.Y., and 15YF1402600 to Y.Z.), the Specialized Research Fund for the Doctoral Program of Higher Education (20100074110010 to Y.Y.), Young Elite Scientists Sponsorship Program by Cast (to Y.Z.), Shanghai Young Top-notch Talent (to Y.Z.), the State Key Laboratory of Bioreactor Engineering (to Y.Y.), the 111 Project (B07023 to Y.Y.), and the Fundamental Research Funds for the Central Universities (to Y.Y. and Y.Z.).

语种：英文

外文关键词：NAD(+); NADH; NADP(+); NADPH; cell metabolism; genetically encoded fluorescent sensors; real-time monitoring; imaging

摘要：Significance: Beyond their roles as redox currency in living organisms, pyridine dinucleotides (NAD(+)/NADH and NADP(+)/NADPH) are also precursors or cosubstrates of great significance in various physiologic and pathologic processes. Recent Advances: For many years, it was challenging to develop methodologies for monitoring pyridine dinucleotides in situ or in vivo. Recent advances in fluorescent protein-based sensors provide a rapid, sensitive, specific, and real-time readout of pyridine dinucleotide dynamics in single cells or in vivo, thereby opening a new era of pyridine dinucleotide bioimaging. In this article, we summarize the developments in genetically encoded fluorescent sensors for NAD(+)/NADH and NADP(+)/NADPH redox states, as well as their applications in life sciences and drug discovery. The strengths and weaknesses of individual sensors are also discussed. Critical Issues: These sensors have the advantages of being specific and organelle targetable, enabling real-time monitoring and subcellular-level quantification of targeted molecules in living cells and in vivo. Future Directions: NAD(+)/NADH and NADP(+)/NADPH have distinct functions in metabolic and redox regulation, and thus, a comprehensive evaluation of metabolic and redox states must be multiplexed with a combination of various metabolite sensors in a single cell. Antioxid. Redox Signal. 28, 213-229.