文献类型：期刊文献

英文题名：Coupling split-lux cassette with a toggle switch in bacteria for ultrasensitive blood markers detection in feces and urine

作者：Zou, Zhen-Ping[1];Yang, Ying[1];Wang, Junshan[2];Zhou, Ying[1];Ye, Bang-Ce[1,3]

机构：[1]East China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Tongji Univ, Shanghai Peoples Hosp 10, Dept Gastroenterol, Shanghai 200072, Peoples R China;[3]Zhejiang Univ Technol, Inst Engn Biol & Hlth, Coll Pharmaceut Sci, Collaborat Innovat Ctr Yangtze River Delta Reg Gre, Hangzhou 310014, Zhejiang, Peoples R China

年份：2022

卷号：214

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20222812339104);WOS:【SCI-EXPANDED(收录号:WOS:000834515200003)】；

基金：Acknowledgments This wok was sponsored by the National Key Research and Devel-opment Program of China (2020YFA0908800) and the National Natural Science Foundation of China (22134003) .

语种：英文

外文关键词：Gene circuit; Whole -cell biosensors; Heme detection; Split; lux cassette; Toggle switches

摘要：Cell-based biosensors have powerful abilities to sense a variety of signal chemical molecules. However, compared with commercialized methods, whole-cell biosensors cannot meet the requirements for the lower sensitivity and faster response. Here, we reprogrammed a gene circuit by coupling split-lux cassette with a toggle switch for detecting heme ultra-sensitively. The resultant biosensor (named YES601) exhibited improved detection limit (0.12 ppm) and satisfied maximum induction ratio (more than 4000 folds) for lysed blood detection. Furthermore, we harnessed YES601 to detect the blood signal in the human urine and feces from the mice with DSSinduced colitis, and the results indicated that YES601 showed more satisfied sensitivity and maximum induction ratio compared with chemical method. This ultrasensitive blood biosensor will be applied to detect trace blood in vitro for early-stage diagnosis of serious diseases, and aiding the rapid development for application in diagnosis in vivo in the future.