文献类型：期刊文献

英文题名：Effect of temperature and surfactant on biomass growth and higher-alcohol production during syngas fermentation by Clostridium carboxidivorans P7

作者：Shen, Shaohuang[1];Wang, Guan[1];Zhang, Ming[1];Tang, Yin[1];Gu, Yang[2];Jiang, Weihong[2];Wang, Yonghong[1];Zhuang, Yingping[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Key Lab Synthet Biol, CAS Ctr Excellence Mol Plant Sci, Shanghai Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China

年份：2020

卷号：7

期号：1

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20224513078386);WOS:【SCI-EXPANDED(收录号:WOS:000586419300002)】；

基金：This study was supported by State Key Laboratory of Bioreactor Engineering, ECUST and CAS-Key Laboratory of Synthetic Biology.

语种：英文

外文关键词：Clostridium carboxidivorans P7; Higher alcohol; Two-step temperature culture; Surfactant; Comparative transcriptome

摘要：Hexanol-butanol-ethanol fermentation from syngas byClostridium carboxidivoransP7 is a promising route for biofuel production. However, bacterial agglomeration in the culture of 37 degrees C severely hampers the accumulation of biomass and products. To investigate the effect of culture temperature on biomass growth and higher-alcohol production,C. carboxidivorans P7 was cultivated at both constant and two-step temperatures in the range from 25 to 37 degrees C. Meanwhile, Tween-80 and saponin were screened out from eight surfactants to alleviate agglomeration at 37 degrees C. The results showed that enhanced higher-alcohol production was contributed mainly by the application of two-step temperature culture rather than the addition of anti-agglomeration surfactants. Furthermore, comparative transcriptome revealed that although 37 degrees C promoted high expression of genes involved in the Wood-Ljungdahl pathway, genes encoding enzymes catalyzing acyl-condensation reactions associated with higher-alcohol production were highly expressed at 25 degrees C. This study gained greater insight into temperature-effect mechanism on syngas fermentation by C. carboxidivorans P7.