文献类型：期刊文献

英文题名：DNA-Based Daisy Chain Rotaxane Nanocomposite Hydrogels as Dual-Programmable Dynamic Scaffolds for Stem Cell Adhesion

作者：Yao, Shengtao[1];Chang, Yongyun[2];Zhai, Zanjing[2];Sugiyama, Hiroshi[3];Endo, Masayuki[3];Zhu, Weiping[1];Xu, Yufang[1];Yang, Yangyang[1];Qian, Xuhong[1,4]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Sch Med, Shanghai Key Lab Orthopaed Implants,Dept Orthopae, Shanghai 200011, Peoples R China;[3]Kyoto Univ, Grad Sch Sci, Dept Chem, Kyoto 6068502, Japan;[4]East China Univ Sci & Technol, State Key Lab Bioreactor, Shanghai 200237, Peoples R China

年份：2022

卷号：14

期号：18

起止页码：20739

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20222012119479);WOS:【SCI-EXPANDED(收录号:WOS:000813090500001)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 31600802) and Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：KEYWORDS; DNA hydrogels; daisy chain rotaxanes; human mesenchymal stem cells; cell adhesion

摘要：mable movements in nanoscales such as sliding, contraction, and regulate the functions of larger length scaled matrix and developing their applications has not yet been reported. Herein we describe the assembly of DNA-based daisy chain rotaxane nanostructure (DNADCR) composed of two hollow DNA nanostructures as macrocycles, two interlocked axles and two triangular prism-shaped DNA structures as stoppers, in which three mechanical states-fixed extended state (FES), sliding state (SS), and fixed contracted state (FCS)-are characterized by using toehold-mediated strand displacement reaction (SDR). The DNA-DCRs are further used as nanocomposites and introduced into hydrogel matrix to produce interlocked hydrogels, which shows modulable stiffness by elongating the interlocked axles to regulate the hydrogel swelling with hybridization chain reaction (HCR) treatment. Then the DCR-hydrogels are employed as dynamic biointerfaces for human mesenchymal stem cells (hMSCs) adhesion studies. First, hMSCs showed lower cell density on bare DCR-hydrogel treated with HCR-initiated swelling for stiffness decreasing. Second, the cell adhesion ligand (RGD) modified DNA-DCRs are constructed for hydrogel functionalization. DCR(RGD) hydrogel endows the mobility of RGDs by switching the mechanical states of DNA-DCR. HMSCs showed increased cell density on DCRSS(RGD) hydrogel than on DCRFCS(RGD) hydrogel. Therefore, our DNA-DCR nanocomposite hydrogel exhibit dual-programmable performances including swelling adjustment and offering sliding for incorporated ligands, which can be both utilized as dynamic scaffolds for regulating the stem cell adhesion. The dual-programmable cross-scale regulation from interlocked DNA nanostructures to hydrogel matrix was achieved, demonstrating a new pathway of DNA-based materials.