文献类型：期刊文献

英文题名：Detection of structured single-strand DNA via solid-state nanopore

作者：Liu, Shao-Chuang[1];Li, Qiao[1];Ying, Yi-Lun[1];Long, Yi-Tao[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn East, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]Nanjing Univ, Sch Chem & Chem Engn, Nanjing, Jiangsu, Peoples R China

年份：2019

卷号：40

期号：16-17

起止页码：2112

外文期刊名：ELECTROPHORESIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000478750500011)】；

语种：英文

外文关键词：DNA detection; Single-molecule analysis; Solid-state nanopore

摘要：Nanopore is a single-molecule analysis method which also employed electrophoresis has achieved promising single-molecule detections. In this study, we designed two kinds of confined spaces by fabricating solid-state nanopores with desirable diameters to study the structured single-strand DNA of C-rich quadruplex. For the nanopore whose diameter is larger than the quadruplex size, the DNA molecule could directly translocate through the nanopore with extremely high speed. For the nanopore whose diameter is smaller than the quadruplex size, DNA molecule which is captured by nanopore could return to the solution without translocation or unzip the quadruplex structure into single-strand and then pass the nanopore. This study certifies that choosing a suitable sensing interface is the vital importance of observing detailed single-molecule information. The solid-state nanopores hold the great potential to study the structural dynamics of quadruplex DNA molecule.