文献类型：期刊文献

英文题名：Synthesis and mass spectra of rearrangement bio-signature metabolites of anaerobic alkane degradation via fumarate addition

作者：Chen, Jing[1,2,5];Zhou, Lei[1,2,5];Liu, Yi-Fan[1,2,5];Hou, Zhao-Wei[3];Li, Wei[3];Mbadinga, Serge Maurice[1,2,5];Zhou, Jing[1,2,5];Yang, Tao[1,2,5];Liu, Jin-Feng[1,2,5];Yang, Shi-Zhong[1,2,5];Wu, Xiao-Lin[3];Gu, Ji-Dong[4];Mu, Bo-Zhong[1,2,5]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]PetroChina, Res Inst Daqing Oilfield Co Ltd, Daqing 163712, Heilongjiang, Peoples R China;[4]Univ Hong Kong, Sch Biol Sci, Pokfulam Rd, Hong Kong, Peoples R China;[5]East China Univ Sci & Technol, Engn Res Ctr Microbial Enhanced Oil Recovery, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2020

卷号：600

外文期刊名：ANALYTICAL BIOCHEMISTRY

收录：;EI(收录号：20201808595878);WOS:【SCI-EXPANDED(收录号:WOS:000537568700006)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 41530318), the Shanghai International Collaboration Program (No. 18230743300), and the Fundamental Research Funds for the Central Universities (No. 222201817017).

语种：英文

外文关键词：Alkylmalonic acids; Rearrangement bio-signature metabolites; Fumarate addition; Mass spectral characteristics; Synthesis of biomarkers

摘要：Metabolite profiling in anaerobic alkane biodegradation plays an important role in revealing activation mechanisms. Apart from alkylsuccinates, which are considered to be the usual biomarkers via fumarate addition, the downstream metabolites of C-skeleton rearrangement can also be regarded as biomarkers. However, it is difficult to detect intermediate metabolites in both environmental samples and enrichment cultures, resulting in lacking direct evidence to prove the occurrence of fumarate addition pathway. In this work, a synthetic method of rearrangement metabolites was established. Four compounds, namely, propylmalonic acid, 2-(2-methylbutyl) malonic acid, 2-(2-methylpentyl)malonic acid and 2-(2-methyloctyl)malonic acid, were synthesized and determined by four derivatization approaches. Besides, their mass spectra were obtained. Four characteristic ions were observed at m/z 133 + 14n, 160 + 28n, 173 + 28n and [INA - (45 + 14n)](+) (n = 0 and 2 for ethyl and n-butyl esters, respectively). For methyl esterification, mass spectral features were m/z 132, 145 and [M - 31](+), while for silylation, fragments were m/z 73, 147, 217, 248, 261 and [M - 15](+). These data provide basis on identification of potential rearrangement metabolites in anaerobic alkane biodegradation via fumarate addition.