文献类型：期刊文献

英文题名：Rapid determination of fumonisin (FB1) by syringe SPE coupled with solid-phase fluorescence spectrometry

作者：Li, Long[1];Chen, Wanchao[1];Li, Hui[2];Iqbal, Jibran[3];Zhu, Ying[1];Wu, Ting[1];Du, Yiping[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Dehong Teachers Coll, Dept Sci & Engn, Xianchi Rd 14, Xianchi Road 678400, Peoples R China;[3]Zayed Univ, Coll Nat & Hlth Sci, Abu Dhabi, U Arab Emirates

年份：2020

卷号：226

外文期刊名：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

收录：;EI(收录号：20194207543432);WOS:【SCI-EXPANDED(收录号:WOS:000504048400056)】；

基金：This work was supported by Science and Technology Commission of Shanghai Municipality (No. 17142201100).

语种：英文

外文关键词：Solid-phase fluorescence; Syringe; SPE; Fumonisin B-1

摘要：Fumonisin B-1 is the most prevalent member of a family of toxins, known as fumonisins, which occurs mainly in maize, wheat and other cereals. Due to its hepatotoxic and nephrotoxic in all animal species, very strict regulations have been imposed on the levels of fumonisin B-1 in cereal and cereal-based foods worldwide. In this work, a rapid determination method of fumonisin B-1 by membrane solid phase extraction coupled with solid-phase fluorescence analysis is developed. A rhodamine based fluorescent probe was used for derivatization with fumonisin B-1. After derivatization and extraction by nylon membrane, the enriched fumonisin B-1 can be detected directly on the membrane without further elution process that is placed in a designed spectra collection device. The established method showed a linear relationship in concentration range of 0.5-5.0 mu g/L, with the R-2 = 0.991, and a limit of detection of 0.119 mu g/L. Method accuracy was further confirmed using LC-MS method by comparing the detection results of 3 corn powder samples spiked with FB1, that demonstrated equivalent results. The results of this study indicated that the proposed method was simple, sensitive, reliable and suitable for trace fumonisins B-1 quantitation in corn-based feeds. (C) 2019 Elsevier B.V. All rights reserved.