文献类型：期刊文献

英文题名：Reversal of multidrug resistance in KB cells with tea polyphenol antioxiclant capacity

作者：Mei, YY; Wei, DZ; Liu, JW

机构：[1]Univ Kentucky, Albert B Chandler Med Ctr, Dept Mol & Cellular Biochem, Lexington, KY 40536 USA;[2]E China Univ Sci & Technol, Inst Biochem, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2005

卷号：4

期号：4

起止页码：468

外文期刊名：CANCER BIOLOGY & THERAPY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000235543900030)】；

语种：英文

外文关键词：multidrug resistance; tea polyphenols; (-)-epi-gallocatechin gallate; doxorubicin; reactive oxygen species

摘要：Tea polyphenol (TP), (-)-epigallocatechin gallate (EGCG) in particular, were examined for their reversal effects on multidrug resistance (MDR) with their antioxidant capacities. TP and EGCG were proved to exert reversal effects on drug resistance KB-A-1 cells by 5.2 and 2.5 times, respectively, but no such effects were found on drug sensitive KB-3-1 cells. With a redox indicator 6-carboxy-2',7'-dichlorodihydrofluorescein (CDCFH), it could be found that the intracellular reactive oxygen species (ROS) decreased in the KB-3-1 cells, which was induced by different concentrations of doxorubicin, whereas the intracellular ROS induced by doxorubicin in KB-A-1 cells maintained a high level. Such observation suggests that drug resistant cells undergo oxidative stress. Furthermore, this elevated redox level in KB-A-1 cells was inhibited by either TP or EGCG. These results demonstrate that the reversal effect of TP and EGCG on MDR acts, at least in part, by regulating the doxorubicin induced intracellular concentration of ROS.