文献类型：期刊文献

英文题名：On-Off Switch of Bioenergetic Channels Regulated by Curcumin-Loaded 3d Composite Scaffold to Steer Bone Regeneration

作者：Lai, Hongchang[1]; Wei, Jianxu[1]; Zhang, Xiaomeng[1]; Zhang, Zhuo[2]; Ding, Xinxin[1]; Li, Yuan[1]; Zhang, Yi[1]; Jiang, Xue[1]; Zhang, Hongbo[2]; Shi, Junyu[1]

机构：[1] Department of Oral Implantology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, China; [2] School of Mechanical and Power Engineering, East China University of Science and Technology, Shanghai, China

年份：2022

外文期刊名：SSRN

收录：EI(收录号：20220285866)

语种：英文

外文关键词：3D printers - Bone - Cell culture - Cell engineering - Defects - Glucose - Scaffolds (biology)

摘要：Metabolic energy to steer osteoblastic differentiation of bone marrow mesenchymal stem cells (BMSCs) could be a promising therapeutic target for bone tissue engineering (BTE), but prior knowledge of this issue is limited. To address bone defects with BTE, we customized a three-dimensional (3D)-printed composite scaffold (Cur@MS) to allow controlled release of curcumin, which could facilitate the "on-off switch" pattern of Glucose transporter 1 (GLUT1) in BMSCs. Consequently, bioenergetic channels, i.e. glucose uptake, were "switched on" to activate GLUT1-RUNX2 crosstalk, which was closely orchestrated with bone regeneration. Furthermore, curcumin-induced cholesterol/lipid raft (Cho/LR) was a "sensor" to trigger the "switch" (GLUT1) by directing its spatial distribution into clusters. In contrast, selective inhibition of Cho/LR and GLUT1 led to a "switch off" pattern and compromised bone regeneration in vivo . Overall, the results suggest Cho/LR is a potential target to steer BMSCs and Cur@MS is an ideal BTE material for stimulating bone regeneration. ? 2022, The Authors. All rights reserved.