文献类型：期刊文献

中文题名：洛伐他汀发酵培养基配方优化及15L罐放大

英文题名：Optimization of medium components for lovastatin production and scale up in 15L bioreactor

作者：吴波[1];陈长华[1];杨琳[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2007

卷号：32

期号：7

起止页码：409

中文期刊名：中国抗生素杂志

外文期刊名：Chinese Journal of Antibiotics

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：响应面设计法;洛伐他汀;土曲霉;发酵

外文关键词：Response surface design; Lovastatin; Aspergillus terreus; Fermentation

摘要：利用响应面方法对土曲霉生产洛伐他汀的培养基进行了优化,使用两水平因子实验对培养基中的碳、氮源组分:葡萄糖、豆粕、蛋白胨、麦精和硝酸钠对洛伐他汀效价的影响进行分析,发现主要的影响因素为葡萄糖和豆粕。通过进一步中心组合实验,考察葡萄糖和豆粕浓度对菌浓、效价、残糖浓度、单位菌体产量(YP/X)和得率(YP/S)的作用。并通过引进残糖浓度为参考,得到最优的葡萄糖和豆粕配比分别为22%和5%,结果比对照提高了17%。同时在15L发酵罐上放大,确定最适初始葡萄糖浓度为21%,效价为7.34g/L。
Response surface design method was used to optimize the medium components for lovastatin produced by Aspergillus terreus. Effects of glucose, soybean meal, peptone, malt extract and NaNO3 on lovastatin productivity were evaluated using a 2-level factorial design. Among the components investigated, glucose and soybean meal played important roles in lovastatin production. A central composite design was used to review the effects of glucose and soybean meal to concentration of biomass, lovastation, and residual glucose respectively, as well as Yp/x and Yp/s. The optimized concentrations of glucose and soybean meal were 22% and 5% respectively, by using residual glucose concentration as a reference, and the optimized medium resulted in a significant increase of lovastatin yield by 17%, as compared with the original one. It was concluded that the optimal concertration of glucose was about 21% and the lovastatin yield was 7.34g/L.