文献类型：期刊文献

英文题名：Revealing and manipulating the LmbU-mediated connection between lincomycin production and the ectABCD gene cluster in S. lincolnensis

作者：Mao, Yue[1];Ye, Jiang[1];Wang, Ruida[2];Wu, Haizhen[1];Zhang, Huizhan[1]

机构：[1]East China Univ Sci & Technol, Bioengn Expt Teaching Demonstrat Ctr, Sch Biotechnol, Dept Appl Biol,State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Tarim Univ, Coll Life Sci & Technol, State Key Lab Incubat Base Conservat & Utilizat Bi, Alar 843300, Peoples R China

年份：2026

卷号：162

起止页码：16

外文期刊名：PROCESS BIOCHEMISTRY

收录：;EI(收录号：20260419949805);WOS:【SCI-EXPANDED(收录号:WOS:001664152800001)】；

基金：This work was funded by the National Natural Science Foundation of China (NSFC) (42406126) and the President's Fund of Tarim University (TDZKBS202536) and the National Key Research and Development Program of China (2021YFC2100600) .

语种：英文

外文关键词：Streptomyces lincolnensis; Lincomycin; LmbU; Ectoine/hydroxyectoine; Osmotic regulation

摘要：Lincomycin, an antibiotic produced by Streptomyces lincolnensis, is widely used to treat Gram-positive bacterial and anaerobic infections. This study found that LmbU, a positive regulatory factor within the lincomycin biosynthesis gene cluster, can positively regulate the extracellular target gene cluster ectABCD (ect), which is involved in the synthesis of ectoine/hydroxyectoine. By knocking out ect, the lincomycin production was increased 2.8-fold. The addition of exogenous L-Asp in both xbl1 and Delta ect strains confirmed that the increased lincomycin production in Delta ect was due to enhanced precursor supply. Furthermore, osmotic stress experiments demonstrated that moderate NaCl concentrations could increase both ect expression and lincomycin production, while higher osmotic pressures resulted in decreased production and biomass. Together, these findings provide new mechanistic insights and offer a theoretical basis for the rational design of high-production industrial strains.