文献类型：期刊文献

中文题名：Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E.coli

作者：Fu-Liang Du[1];Hui-Lei Yu[1];Jian-He Xu[1];Chun-Xiu Li[1]

机构：[1]Laboratory of Biocatalysis and Synthetic Biotechnology,State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,P.R.China

年份：2014

卷号：1

期号：1

起止页码：164

中文期刊名：Bioresources and Bioprocessing

外文期刊名：生物资源与生物加工(英文)

基金：financially supported by the National Natural Science Foundation of China(No.21276082);Ministry of Science and Technology,P.R.China(Nos.2011CB710800);Shanghai Commission of Science and Technology(No.11431921600).

语种：英文

中文关键词：Limonene;Biosynthesis;Escherichia coli;MEP pathway;Geranyl pyrophosphate synthase;Limonene synthase;Two-phase culture system

摘要：Background:Limonene is an important monoterpene used as a chemical commodity and precursor for producing biofuels,flavor and medicinal compounds.Results:In this paper,we engineered Escherichia coli by embedding two exogenous genes encoding a limonene synthase(LS)and a geranyl diphosphate synthase(GPPS)for production of limonene.Out of 12 E.coli strains transformed with various plasmids,the best one with p15T7-ls-gpps produced limonene with a titer of 4.87 mg/L.In order to enhance the limonene production,two rate-limiting enzymes in the endogenous MEP pathway of E.coli,1-deoxy-xylulose-5-phosphate synthase(DXS)and isopentenyl diphosphate isomerase(IDI),were overexpressed consecutively on vector pET21a+,resulting in a production of 17.4 mg_(limonene)/L at 48 h.Conclusions:After the preliminary optimization of the medium in a two-phase culture system composed of n-hexadecane(1/50,V_(org)/V_(aq)),the final production of limonene was raised up to 35.8 mg/L,representing approximately a 7-fold improvement compared to the initial titer.