文献类型：期刊文献

中文题名：甘露聚糖肽的结构鉴定

英文题名：Structural Characterization of Mannatide

作者：沈艳红[1];陈林枭[1];张文清[1];徐志珍[1];宛燕飞[2];张力[2];夏玮[1]

机构：[1]华东理工大学上海市功能性材料化学重点实验室,上海200237;[2]成都利尔药业有限公司,成都610083

年份：2016

卷号：37

期号：6

起止页码：1088

中文期刊名：高等学校化学学报

外文期刊名：Chemical Journal of Chinese Universities

收录：CSTPCD;;EI(收录号：20162602532970);Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000377534400010)】；北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：Supported by the Scientific and Technological Achievement Transformation Project of Sichuan Province, China(No. 2012SC0024).

语种：中文

中文关键词：甘露聚糖肽;多糖;结构

外文关键词：Mannatide; Polysaccharide; Structure;

摘要：采用离子交换柱层析和凝胶柱层析从甘露聚糖肽原料中分离纯化得到4种均一糖肽MT1-A,MT1-B,MT2-A和MT2-B;通过单糖组成分析、甲基化分析、氢核磁共振谱(~1H NMR)、红外光谱(IR)和氨基酸组成分析等手段对均一糖肽MT1-A,MT1-B和MT2-B的结构进行了鉴定.结果表明,3种均一糖肽的单糖组成、糖残基的连接方式与MT2-A相同,但是分子量以及氨基酸总量有所不同.测定了不同批次甘露聚糖肽原料的~1H NMR谱,以MT2-A的~1H NMR谱图为标准谱,以异头氢区域为鉴定区域,通过计算不同批次甘露聚糖肽原料以及其它3种均一糖肽与MT2-A的~1H NMR谱的相关系数,定量评价了不同批次甘露聚糖肽原料与4种均一糖肽的相似程度.结果表明,甘露聚糖肽是由糖链结构一致、分子量和氨基酸含量不同的几种均一糖肽构成的混合物.
DEAE-52 cellulose column and Sephacryl S-300 gel column were used to isolate and purify mannatide to give four homogeneous glycopeptides termed as MT1-A,MT1-B,MT2-A and MT2-B. The chemical structure of MT1-A,MT1-B and MT2-B were elucidated using monosaccharide composition analysis,methylation analysis,~1H-nuclear magnetic resonance（~1H NMR） spectroscopy,infrared（ IR） spectroscopy and amino acids analysis. It was concluded that the monosaccharide composition and connection type of sugar residues of MT1-A,MT1-B and MT2-A were the same as MT2-A reported before while the molecular weight and amino acid content of four homogeneous glycopeptides were slightly different. The^1H NMR spectrum of MT2-A was regarded as the standard reference spectrum and the anomeric region of each spectrum（ δ 4. 89—5. 90） was selected as the identification region. The ~1H NMR spectra of MT1-A,MT1-B,MT2-A and different batches of mannatide were compared with the standard reference spectrum,using the correlation coefficient to quantitatively calculate the similarity. The results showed that mannatide was a mixture of several kinds of homogeneous glycopeptides with the same glycan structure but different molecular weight and amino acid content.