文献类型：期刊文献

英文题名：Bio-inspired redox-cycling antimicrobial film for sustained generation of reactive oxygen species

作者：Liu, Huan[1];Qu, Xue[1];Kim, Eunkyoung[2];Lei, Miao[1];Dai, Kai[1];Tan, Xiaoli[4];Xu, Miao[5,6];Li, Jinyang[2];Liu, Yangping[4];Shi, Xiaowen[3];Li, Peng[5,6];Payne, Gregory F.[2];Liu, Changsheng[1]

机构：[1]East China Univ Sci & Technol, Key Lab Ultrafine Mat, State Key Lab Bioreactor Engn, Minist Educ, Shanghai 200237, Peoples R China;[2]Inst Biosyst & Biotechnol Res & Fischell, Dept Bioengn, 5115 Plant Sci Bldg, College Pk, MD 20742 USA;[3]Wuhan Univ, Dept Environm Sci, Coll Resource & Environm Sci, Wuhan 430072, Hubei, Peoples R China;[4]Tianjin Med Univ, Sch Pharm, Tianjin Key Lab Technol Enabling Dev Clin Therape, Tianjin 300070, Peoples R China;[5]Nanjing Tech Univ NanjingTech, Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, KLOFE, Nanjing 210009, Jiangsu, Peoples R China;[6]Nanjing Tech Univ NanjingTech, Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, IAM, Nanjing 210009, Jiangsu, Peoples R China

年份：2018

卷号：162

起止页码：109

外文期刊名：BIOMATERIALS

收录：;EI(收录号：20180704792407);WOS:【SCI-EXPANDED(收录号:WOS:000428097800009)】；

基金：The support from the National Natural Science Foundation of China (51621002, 51573047), the 111 project (B14018), the Fundamental Research Funds for the Central Universities (222201718002, 222201717002, 222201717015), and the United States National Science Foundation (CBET-1435957) and Defense Threat Reduction Agency (HDTRA1-13-0037).

语种：英文

外文关键词：Antimicrobial; Bio-inspiration; Catechol; Chitosan; Reactive oxygen species; Redox-activity

摘要：Open wounds and burns are prone to infection and there remains considerable interest in developing safe and effective mechanisms to confer antimicrobial activities to wound dressings. We report a bio-mimetic wound dressing for the in situ and sustained generation of reactive oxygen species (ROS). Specifically, we fabricate a catechol-modified chitosan film that mimics features of the melanin capsule generated during an insect immune response to infection. We use an electrochemical reverse engineering approach to demonstrate that this catechol-chitosan film possesses redox-activities and can be repeatedly oxidized and reduced. In vitro tests demonstrate that this film catalyzes the transfer of electrons from physiological reductant ascorbate to O-2 for sustained ROS generation, and confers ascorbate-dependent antimicrobial activities. In vivo antimicrobial experiment with a rat subcutaneous model indicates the catechol-chitosan film at reduced state inhibits the bacterial growth and alleviates the infection of the incisions. Open wound healing tests with a mouse model indicate that the catechol-chitosan film suppresses the bacterial population at the wound site, induces less inflammation and promotes wound healing. We envision this biomimetic approach for the sustained, localized and in situ generation of ROS could provide new opportunities for wound management by protecting against pathogen infection and potentially even enlisting ROS-mediated wound healing mechanisms. (C) 2018 Elsevier Ltd. All rights reserved.