文献类型：期刊文献

英文题名：Optimizing the Chemical Recognition Process of a Fluorescent Chemosensor for α-Ketoglutarate

作者：He, Ye;Guo, Zhiqian[1];Jin, Pengwei;Jiao, Changhong;Tian, He;Zhu, Weihong

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Inst Fine Chem, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China

年份：2015

卷号：54

期号：11

起止页码：2886

外文期刊名：INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH

收录：;EI(收录号：20151300695182);WOS:【SCI-EXPANDED(收录号:WOS:000351972600006)】；

基金：This work was supported by the National 973 Program (2013CB733700), NSFC for Creative Research Groups (21421004) and Distinguished Young Scholars (21325625), NSFC/China, Oriental Scholarship, Shanghai Pujiang Program (13PJD010), Fok Ying Tong Education Foundation (142014), and Fundamental Research Funds for the Central Universities (222201313010).

语种：英文

外文关键词：Chemosensor - Diagnosis - Physiology - Body fluids - Fluorescence - Reaction kinetics

摘要：alpha-ketoglutarate (alpha-ICA) can convert to 2-hydroxygiutarate (24-HG), which is confitmecr to be associated with many diseases, especially with acute myeloid leukemia (AML). In this paper, a novel reaction-based chemosensor DT based on the typical Schiff-base reaction was designed for sensing the biomarker of alpha-KA, in which a diazanyl group as the recognition group was-linked with a benzothiadiazole unit as the fluorophore moiety. Considering the typical Scbiff-base reaction to generate hydrazones suffering from slow kinetics, particularly under neutral conditions,,a series Of parallel-exp: eriments was canducted for optimizing the chemical recognition process, including varying the solvent, -reaction temperature, reactant concentration, and reaction rate. The optimum condition was established as a pH value; temperature, alpha-ICA:concentration, and response time of 5.7, 30 degrees C; 100 mu M, and 20 min, respectively. Notably, in contrast withthe initial 6:3-fold fluorescence enharidement, the remarkable 75-fold fluorescence enhancement ((I - I-0)/I-0 at 560 nm) was observed by-optimizing the chemical recognition process of DT and alpha-ICA Finally, DT was carried out for the chemical recognition, processing of alpha-KA in serum, We demonstrated that DT is selective for alpha-KA over other potential biologically interferences with similar structures and thus is suitable for detecting alpha-ICA in serum. On the basis of the Optimized chemical recognition process, DT shows high potential application for sensing alpha-KA with remarkable fluorescence enhancement. This work provided a potential Method that is quick and convenient for sensing biomarker alpha-KA in serum. It is worth rioting that without complicated pretreatment, utilizing a novel reaction-based fluorescent chemosensor may establish a new promising platform for clinical diagnosis Biomarker: