文献类型：期刊文献

英文题名：An improved 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay for evaluating the viability of Escherichia coli cells

作者：Wang, Hengwei[1,2];Cheng, Hairong[2];Wang, Fengqing[1];Wei, Dongzhi[1];Wang, Xuedong[1]

机构：[1]E China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Minist Educ, Key Lab Microbial Metab, Shanghai 200240, Peoples R China

年份：2010

卷号：82

期号：3

起止页码：330

外文期刊名：JOURNAL OF MICROBIOLOGICAL METHODS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000282113000021)】；

基金：This study was supported by a grant (No. 2009ZX09306-008) from the National Key Technologies R&D Programs of the Ministry of Science and Technology of China.

语种：英文

外文关键词：MTT reduction; Formazan; E. coli; Viability

摘要：To reduce the interference in MTT reduction assay, we developed an improved protocol for evaluating the viability of Escherichia coli cells by conducting MTT reduction in 1.5-mL centrifuge tubes and formazan dissolution in test tubes. Our study is helpful in developing protocols for measuring the viability of other gram-negative bacteria. (C) 2010 Elsevier B.V. All rights reserved.