文献类型：期刊文献

英文题名：Engineering Mycolicibacterium neoaurum for the production of antioxidant ergothioneine

作者：Xiong, Liang-Bin[1,2,3];Xie, Zhi-Yong[1];Ke, Jie[1];Wang, Li[1];Gao, Bei[1];Tao, Xin-Yi[1];Zhao, Ming[1];Shen, Ya-Ling[1];Wei, Dong-Zhi[1];Wang, Feng-Qing[1]

机构：[1]East China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Univ Med & Hlth Sci, Collaborat Innovat Ctr Biomed, Jiading Dist Cent Hosp Affiliated, Shanghai, Peoples R China;[3]Huawei Safety Evaluat & Med Res Shanghai Co Ltd, Shanghai, Peoples R China

年份：2022

卷号：1

期号：1

起止页码：26

外文期刊名：FOOD BIOENGINEERING

收录：EI(收录号：20232414215004);WOS:【ESCI(收录号:WOS:001332182200003)】；

基金：National Key Research and Development Program of China, Grant/Award Number: SQ2020YFC210061; National Natural Science Foundation of China, Grant/Award Numbers: 21776075, 32100067; Natural Science Foundation of Shanghai, Grant/Award Number: 20ZR1415100

语种：英文

外文关键词：endogenous metabolic engineering; ergothioneine; Mycolicibacterium neoaurum; S-adenosyl-L-methionine regeneration

摘要：Ergothioneine (EGT) represents valuable protective functions for humans, but EGT from the diet cannot meet daily requirements. Although the heterologous synthesis of EGT had been achieved, it is still a challenge to obtain stable and high-yield EGT-producing cell factories. Here, after the co-overexpression of the EGT synthetic gene cluster and hisG, hisC, and allB1 in Mycolicibacterium neoaurum, the natural EGT titer was increased by 7.2-folds. However, the degradation problem of EGT in large-scale fermentation needs to be urgently solved. A putative lyase gene Mn_3042 was inactivated, thus inhibiting the product degradation and increasing the EGT titer by 21%. Moreover, the enhancement of S-adenosyl-L-methionine regeneration further increased EGT titer by 28%. After optimization of fed-batch fermentation, the yield of EGT was boosted to 1.56g/L with a productivity of 7.2mg/L/h. This study provides a systematic engineering strategy for developing EGT-producing cell factories.