文献类型：期刊文献

英文题名：Meta-Omics Analysis of a Formate Sidestream-Mediated Partial Nitritation-Coupled Anammox Process

作者：Wang, Jipeng[1,2];Yan, Xinjie[1];Zhuang, Jinlong[3];Liu, Yongdi[1];Li, Wei[1,4,5]

机构：[1]East China Univ Sci & Technol, Natl Engn Res Ctr Ind Wastewater Detoxicat & Reso, Shanghai 200237, Peoples R China;[2]Changzhou Univ, Sch Environm Sci & Engn, Changzhou 213164, Jiangsu, Peoples R China;[3]Suzhou Univ Sci & Technol, Sch Environm Sci & Engn, Suzhou 215009, Jiangsu, Peoples R China;[4]Shanghai Inst Pollut Control & Ecol Secur, Shanghai 200092, Peoples R China;[5]Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing 210023, Jiangsu, Peoples R China

年份：2023

卷号：3

期号：2

起止页码：510

外文期刊名：ACS ES&T WATER

收录：WOS:【ESCI(收录号:WOS:000916248900001)】；

基金：This work was supported by National Natural Science Foundation of China (52170076), Shanghai Rising-Star Program (20QC1400500), State Key Laboratory of Pollution Control, and Resource Reuse (PCRRF20024).

语种：英文

外文关键词：formic acid; partial nitritation; anammox; metagenomics; metatranscriptomics

摘要：A stable partial nitritation process that was achieved by treating activated sludge in sidestream with formate as a selective inhibitor has been previously described. However, little is known about the effect of formate on the microbial communities in both the partial nitritation and the subsequent anammox process. To this end, a formate sidestream-mediated partial nitritationcoupled anammox process was established and studied. The effluent ratio of nitrite/ammonia in the partial nitritation remained at 1.24 +/- 0.19, and the total nitrogen removal efficiency of the anammox process was maintained at 80.0 +/- 1.6%. Metagenomic and metatranscriptomic analysis showed that the nitrite oxidizing bacteria (NOB) was almost eliminated from the partial nitritation process, while Nitrosomonas europaea was the dominant ammonia-oxidizing bacteria (AOB) with an abundance of 30.8% of total reads. The high expression of nitrous oxide reductase genes in the partial nitritation process suggested a great potential for mitigating nitrous oxide release. Candidatus Kuenenia stuttgartiensis and Candidatus Brocadia sinica were identified as the main anammox bacteria, and their abundances were as high as 49.3 and 11.8%, respectively. There was a highly expressed gene encoding a formate tetrahydrofolate ligase in the Candidatus Kuenenia stuttgartiensis metagenome-assembled genomes (MAG), suggesting that formaterelated metabolic activity may contribute to its enrichment in the anammox process.