文献类型：期刊文献

英文题名：Nanoscale Metal-Organic Frameworks for Ratiometric Oxygen Sensing in Live Cells

作者：Xu, Ruoyu[1];Wang, Youfu[1,2];Duan, Xiaopin[1];Lu, Kuangda[1];Micheroni, Daniel[1];Hu, Aiguo[2];Lin, Wenbin[1]

机构：[1]Univ Chicago, Dept Chem, 929 East 57th St, Chicago, IL 60637 USA;[2]E China Univ Sci & Technol, Sch Mat Sci & Engn, Shanghai Key Lab Adv Polymer Mat, Shanghai 200237, Peoples R China

年份：2016

卷号：138

期号：7

起止页码：2158

外文期刊名：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

收录：;EI(收录号：20161002049688);WOS:【SCI-EXPANDED(收录号:WOS:000371103900022)】；

基金：We thank the NIH (UO1-CA198989 and P30 CA014599) for funding support and Dr. Vytas Bindokas for help with confocal microscopy imaging. Additional support was provided by the Chicago MRSEC, which is funded by NSF through grant DMA-1420709.

语种：英文

外文关键词：Ligands - Nanotechnology - Crystalline materials - Platinum compounds - Organometallics

摘要：We report the design of a phosphorescence/fluorescence dual-emissive nanoscale metal-organic framework (NMOF), R-UiO, as an intracellular oxygen (O-2) sensor. R-UiO contains a Pt(II)-porphyrin ligand as an O-2-sensitive probe and a Rhodamine-B isothiocyanate ligand as an O-2-insensitive reference probe. It exhibits good crystallinity, high stability, and excellent ratiometric luminescence response to O-2 partial pressure. In vitro experiments confirmed the applicability of R-UiO as an intracellular O-2 biosensor. This work is the first report of a NMOF-based intracellular oxygen sensor and should inspire the design of ratiometric NMOF sensors for other important analytes in biological systems.