文献类型：期刊文献

英文题名：Using an F₂ screen to monitor frequency of resistance alleles to Bt cotton in field Populations of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae)

作者：Xu, Zhiping[2,5];Liu, Fengyi[2];Chen, Jin[2];Huang, Fangneng[3];Andow, David A.[4];Wang, Yanhua[2];Zhu, Yu Cheng[1];Shen, Jinliang[2]

机构：[1]USDA ARS, JWDSRC, Stoneville, MS 38776 USA;[2]Nanjing Agr Univ, Coll Plant Protect, Dept Pesticide Sci, Nanjing 210095, Peoples R China;[3]Louisiana State Univ, AgCtr, Dept Entomol, Baton Rouge, LA 70803 USA;[4]Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA;[5]E China Univ Sci & Technol, Inst Pesticides & Pharmaceut, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China

年份：2009

卷号：65

期号：4

起止页码：391

外文期刊名：PEST MANAGEMENT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000264520100008)】；

基金：The authors are grateful to Qiuxian County Plant Protection Station for providing facilities and field data of bollworm occurrence. Special thanks are due to Dr Xueming Liu (Kansas State University, Manhattan, KS) and Dr Huarong Li (Iowa State University, Ames, IA) for their valuable comments and suggestions for improving an early version of this manuscript. This research was supported by Special Funding of Transgenic Plant Study and Industrialization Opening-up and Development (J00-C-002) and the National Scientific Research Fund (30270889).

语种：英文

外文关键词：Helicoverpa armigera; F-2 screen; Bt cotton; Bacillus thuringiensis; resistance; allele

摘要：BACKGROUND: In an effort to control the most devastating cotton pest, Helicoverpa armigera (Hubner), in Qiuxian County (Hebei, China), Bt cotton has been planted almost exclusively since 1998 in this area. Owing to the high insecticidal selection pressures in this region, monitoring of Bt resistance in H. armigera is necessary so that proactive actions can be implemented before field control measures fail. From 2003 to 2005, an F-2 screen was conducted in order to monitor Bt resistance in H. armigera populations collected from this area. RESULTS: The F-2 screen showed that 15 out of 278 isofemale lines carried resistance alleles to Bt cotton. The resistance allele frequency in field populations of H. armigera ranged from 0.0119 to 0.0297, with an overall frequency of 0.0146 and a 95% confidence interval of 0.0084-0.0225 for the 3 year period. This value is greater than the value reported from 1999 (P < 0.05). CONCLUSION: A fluctuating but overall increase in resistance allele frequency was detected in the field populations of H. armigera in Qiuxian County from 1999 to 2005. To prevent further increases in Bt resistance frequency in this pest, it is necessary to introduce Bt cotton expressing multiple Bt toxins and integrate this technology with other tactics for management of this key pest. (C) 2009 Society of Chemical Industry