文献类型：期刊文献

英文题名：Low-temperature-active and salt-tolerant β-mannanase from a newly isolated Enterobacter sp strain N18

作者：You, Jia[1,2];Liu, Jin-Feng[1,2];Yang, Shi-Zhong[1,2];Mu, Bo-Zhong[1,2,3]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China;[3]Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2016

卷号：121

期号：2

起止页码：140

外文期刊名：JOURNAL OF BIOSCIENCE AND BIOENGINEERING

收录：;EI(收录号：20152801023442);WOS:【SCI-EXPANDED(收录号:WOS:000370887300002)】；

基金：This work was supported by the National Natural Science Foundation of China (31200101), the 863 Program (2013AA064403).

语种：英文

外文关键词：Enterobacter; beta-Mannanase; Low-temperature-active; Salt-tolerant; Viscosity

摘要：A low-temperature-active and salt-tolerant beta-mannanase produced by a novel mannanase-producer, Enterobacter sp. strain N18, was isolated, purified and then evaluated for its potential application as a gel-breaker in relation to viscosity reduction of guar-based hydraulic fracturing fluids used in oil field. The enzyme could lower the viscosity of guar gum solution by more than 95% within 10 min. The purified beta-mannanase with molecular mass of 90 kDa displayed high activity in a broad range of pH and temperature: more than 70% of activity was retained in the pH range of 3.0-8.0 with the optimal pH 7.5, about 50% activity at 20 degrees C with the optimal temperature 50 degrees C. Furthermore, the enzyme retained >70% activity in the presence of 0.5-4.0 M NaCl. These properties implied that the enzyme from strain N18 had potential for serving as a gel-breaker for low temperature oil wells and other industrial fields, where chemical gel breakers were inactive due to low temperature. (C) 2015, The Society for Biotechnology, Japan. All rights reserved.