文献类型：期刊文献

英文题名：A New Strategy for the Enzymatic Degradation of Sudan Dyes in Non-alkaline Conditions

作者：Min, Liang[1];Zhang, Lei[1];Jiang, Xin[2];Wei, Wei[1];Wei, Dongzhi[1];Wang, Wei[1]

机构：[1]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, POB 311,130 Meilong Rd, Shanghai 200237, Peoples R China;[2]TEDA, Tianjin Hualida Biotechnol Co Ltd, 203 Huanghai Rd, Tianjin, Peoples R China

年份：2016

卷号：227

期号：6

外文期刊名：WATER AIR AND SOIL POLLUTION

收录：;EI(收录号：20162302475231);WOS:【SCI-EXPANDED(收录号:WOS:000377456000023)】；

基金：This research was supported by the National Natural Science Foundation of China (No. C010302-31500066), the National Basic Research Program of China (973, Program No. 2012CB721103), and the National High Technology Research and Development Program of China (863, Program No. 2013AA102109).

语种：英文

外文关键词：Sudan dye; Horseradish peroxidase; Glucose oxidase; Phenols; Inactivation; Co-expression

摘要：Sudan dyes are widely used as coloring agents in various solvents, waxes, and polishes. However, the dyes are environmental contaminants and Sudan I is a weak carcinogen, and its removal from wastewater remains challenging. Here, we developed a new strategy for Sudan dye degradation for use in the non-alkaline conditions typically found in wastewater. By combing glucose oxidase (GOD) and horseradish peroxidase (HRP), we avoided the hydrogen peroxide-induced HRP damage and inactivation. Moreover, the GOD-HRP-coupled degradation of Sudan dyes were enhanced by the addition of different kinds of phenols. Systematic investigations were carried out to determine the optimal process parameters (i.e., phenol concentration, pH value, temperature, and enzyme dose) for degrading Sudan 1 with GOD and HRP. Also, this strategy could be applied to degradation of Sudan II and Sudan III. We were also able to co-express GOD and HRP in a prokaryotic-like polycistronic expression system in Pichia pastoris, based on the internal ribosome entry sites (IRES). Therefore, this fermented liquid containing GOD and HRP might be used in the future to degrade pollutants in weakly acidic conditions.