文献类型：期刊文献

英文题名：A gel-slit method for quick and gentle platelet-rich plasma separation

作者：Luo, Lexi[1];Cao, Hongliang[2];Qin, Hui[3];Cao, Huiliang[1,4,5]

机构：[1]East China Univ Sci & Technol, Sch Mat Sci & Engn, Interfacial Electrochem & Biomat, Shanghai 200237, Peoples R China;[2]Shanghai Yiya Med Technol Co LTD, Shanghai 201712, Peoples R China;[3]Shanghai Jiao Tong Univ, Dept Orthopaed, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R China;[4]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China;[5]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China

年份：2025

卷号：364

外文期刊名：SEPARATION AND PURIFICATION TECHNOLOGY

收录：;EI(收录号：20251118052293);WOS:【SCI-EXPANDED(收录号:WOS:001448018600001)】；

基金：This work was jointly supported by grants from the National Natural Science Foundation of China (32271399 and 31870945) , the Natural Science Foundation of Shanghai (21ZR1415700) , and the Shanghai Committee of Science and Technology (23S31901700) .

语种：英文

外文关键词：Sodium alginate; Centrifugation; Calcium; Blood; Platelet concentrates

摘要：Platelet-rich plasma (PRP) is widely used in various medical treatments; however, its clinical effect is scattered because of time-consuming separations. Here, a sodium alginate (SA)-based thixotropic gel was produced by internal gelation of calcium, yielding a "gel-slit" dependent quick (within 4 min) and gentle (135 g) PRP separation. By analyzing the rheological properties of gels with different SA concentrations and calcium cross-linking degrees, it was found that the gels' viscosity thinning rate (reaching a proper viscosity under centrifugal shear for red blood cell sedimentation) during shearling (or centrifugation) is a key parameter determining the separation efficacy and PRP quality. A thixotropic gel, made from 8 % of SA and a calcium cross-linking degree of 0.4, has an optimal viscosity thinning rate (the viscosity thins 63.99 f 0.31 % to reach a viscosity of 53.38 f 0.95 Pa & sdot;s) for quick PRP separation at low centrifugation speed. By spinning at 1100 revolutions per minute (135 g) for merely 4 min, the gel enables high yield (production of 1.32 f 0.03 mL PRP from 3 mL of whole blood) and high platelet capture efficiency (80.84 f 1.31 %) PRP separation (the platelet concentration was1.84 f 0.02 times that of the original blood). This study provides new insights into the mechanism of thixotropic gel-assisted PRP separation for more convenient PRP treatments.