文献类型：期刊文献

英文题名：An Aie-Active Fluorescent Probe with Near-Infrared (Nir) Emission for Selectively Monitoring and Bioimaging Cys in Living Cells

作者：Wang, Cheng-Yun[1]; Zhang, Zhen[1]; Zhao, Weijun[1]; Chen, Yun[1]; Wei, Sheng-Hao[1]; Zhu, Fei[1]; Zhu, Xu-Zhong[1]

机构：[1] Key Laboratory for Advanced Materials, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China

年份：2023

外文期刊名：SSRN

收录：EI(收录号：20230102942)

语种：英文

外文关键词：Amino acids - Biosynthesis - Cytology - Fluorescence - Fluorescence spectroscopy - Infrared devices - Lanthanum compounds - Metabolism - Probes

摘要：Cysteine (Cys), as a kind of essential bio-thiols, has a pivotal role in modulating cell metabolism, maintaining intracellular redox balance and facilitating protein synthesis. To investigate its role in these physiological processes, fluorescent probe for selectively detecting Cys is one of important methods. However, most of the probes have ACQ effect, which limits their applications in the field of biosensing and bioimaging. Herein, an AIE-active fluorescent probe TCF-TPA was synthesized for specifically detecting Cys in HeLa cells. TCF-TPA, consisted of a tricyanofuran (TCF) fluorophore, was modified with an acrylate group for distinguish Cys from other bio-thiols. The incorporation of electron-donating triphenylamine endowed the probe with AIE property and strong intramolecular charge transfer effect, thereby enabling the probe to have near-infrared (NIR) emission length (666 nm). Meanwhile, TCF-TPA possessed low detection limit for Cys (73.0 nM) and was successfully performed bioimaging of Cys in HeLa cells. ? 2023, The Authors. All rights reserved.