文献类型：期刊文献

英文题名：Isolation of Rhodococcus sp. strain ECU0066, a new sulfide monooxygenase-producing strain for asymmetric sulfoxidation

作者：Li, Ai-Tao[1]; Zhang, Jian-Dong[1]; Xu, Jian-He[1,3]; Lu, Wen-Ya[2]; Lin, Guo-Qiang[2]

机构：[1] Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; [2] Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China; [3] Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China

年份：2009

卷号：75

期号：2

起止页码：551

外文期刊名：Applied and Environmental Microbiology

收录：EI(收录号：20090311864842)

语种：英文

外文关键词：Sulfur compounds - Enzyme activity

摘要：A new and efficient sulfide monooxygenase-producing strain, ECU0066, was isolated and identified as a Rhodococcus sp. that could transform phenylmethyl sulfide (PMS) to (S)-sulfoxide with 99% enantiomeric excess via two steps of enantioselective oxidations. Its enzyme activity could be effectively induced by adding PMS or phenylmethyl sulfoxide (PMSO) directly to a rich medium at the early log phase (6 h) of fermentation, resulting in over 10-times-higher production of the enzyme. This bacterial strain also displayed fairly good activity and enantioselectivity toward seven other sulfides, indicating a good potential for practical application in asymmetric synthesis of chiral sulfoxides. Copyright ? 2009, American Society for Microbiology. All Rights Reserved.