文献类型：期刊文献

英文题名：A Structural Study of Escherichia coli Cells Using an In Situ Liquid Chamber TEM Technology

作者：Wang, Yibing[1];Chen, Xin[2,3,4];Cao, Hongliang[2,3];Deng, Chao[2,3];Cao, Xiaodan[1];Wang, Ping[1]

机构：[1]E China Univ Sci & Technol, Biomed Nanotechnol Ctr, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Sch Mat Sci & Engn, Shanghai Key Lab Adv Polymer Mat, Shanghai 200237, Peoples R China;[4]Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, State Key Lab Funct Mat Informat, Shanghai 200050, Peoples R China

年份：2015

卷号：2015

外文期刊名：JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000349777900001)】；

基金：This work was supported by the Major Programs of Ministry of Education of China and National Natural Science Foundation of China (21303050 and 31471659), the China Postdoctoral Science Foundation (2013M540334), the Science and Technology Commission of Shanghai Municipality Project (11 nm0507000), the Shanghai Leading Academic Discipline Project (B502), and the SRF for ROCS, SEM.

语种：英文

摘要：Studying cell microstructures and their behaviors under living conditions has been a challenging subject in microbiology. In this work, in situ liquid chamber TEM was used to study structures of Escherichia coli cells in aqueous solutions at a nanometer-scale resolution. Most of the cells remained intact under electron beam irradiation, and nanoscale structures were observed during the TEM imaging. The analysis revealed structures of pili surrounding the E. coli cells; themovements of the pili in the liquid were also observed during the in situ tests. This technology also allowed the observation of features of the nucleoid in the E. coli cells. Overall, in situ TEM can be applied as a valuable tool to study real-time microscopic structures and processes in microbial cells residing in native aqueous solutions.