文献类型：期刊文献

英文题名：Highly Selective Fluorescent Turn-On Probe for Protein Thiols in Biotin Receptor-Positive Cancer Cells

作者：Sun, Qian[1];Sun, Deheng[2];Song, Lun[1];Chen, Zhuo[2];Chen, Zhaoyang[1];Zhang, Weibing[1];Qian, Junhong[1]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2016

卷号：88

期号：6

起止页码：3400

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20161202141186);WOS:【SCI-EXPANDED(收录号:WOS:000372391500058)】；

基金：This work was financially supported by NSFC (Nos. 21576085 and 21475044) and Shanghai Municipal Natural Science Foundation (15ZR1409000). We appreciate Prof. Y. Yang (ECUST) for improving our paper.

语种：英文

外文关键词：Lanthanum compounds - Body fluids - Coenzymes - Fluorescence imaging - Cytology - Cancer cells - Diseases

摘要：Sulfhydryl-containing proteins play critical roles in various physiological and biological processes, and the activities of those proteins have been reported to be susceptible to thiol oxidation. Therefore, the development of protein thiol target fluorescent probe is highly desirable. In the present work, a biotinylated coumarin fluorescence "off-on" probe SQ for selectively detecting protein thiols in biotin receptor-positive cancer cells was designed with a 2,4-dinitrobenzenesulfony as the thiol receptor. The probe exhibited dramatic fluorescence responses toward sulfhydryl-containing proteins (ovalbumin (OVA), bovine serum albumin (BSA)): up to 170-fold fluorescence enhancement with 70 nm blue-shift was observed with the addition of OVA. However, low molecular weight thiols (Cys, glutathione (GSH), Hcy) caused negligible fluorescence changes of SQ, In addition, biotin receptor-positive Hela cells displayed strong red and green fluorescence after incubation of SQ for 1 h; neither red nor green fluorescence signal could be visualized in biotin-negative normal lung Wi38 cells. These results imply that the probe has potential application in fluorescent imaging protein thiols on the:surface of Hela cells.