文献类型：期刊文献

英文题名：Characterization of C-glycosyl quinochalcones in Carthamus tinctorius L. by ultraperformance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry

作者：Jin, Yu[1]; Zhang, Xiu-Li[1]; Shi, Hui[2]; Xiao, Yuan-Sheng[1]; Ke, Yan-Xiong[2]; Xue, Xing-Ya[1]; Zhang, Fei-Fang[1]; Liang, Xin-Miao[1,2,3]

机构：[1] Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China; [2] School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China; [3] Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China

年份：2008

卷号：22

期号：8

起止页码：1275

外文期刊名：Rapid Communications in Mass Spectrometry

收录：EI(收录号：20231613925829)

语种：英文

外文关键词：Carbon - Glucose - Mass spectrometry - Negative ions - Positive ions

摘要：C-Glycosyl quinochalcones are unique components in Carthamus tinctorius L. The reported C-glycosyl quinochalcones have the same quinochalcone skeleton with a hydroxyl group at the 5′-position and a glucose linked to this position with a carbon-carbon bond. In this study, the standard hydroxysafflor yellow A and water-extracted fraction of Carthamus tinctorius L. were analyzed by ultraperformance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOFMS) in both positive and negative ion modes. The fragmentation pathways of C-glycosyl quinochalcones were interpreted and validated by accurate mass measurement. Their fragmentation showed a special cleavage at the C-C bond except for the typical internal cleavage at the sugar moiety of other C-glycosyl flavonoids. In positive ion mode, cleavage of the 5′-glucose produced an [M+H-162]+ ion by a neutral loss, while cleavage of the 5′-glucose in negative ion mode led to an [M-H-163] -. ion by radical cleavage. The cleavage from the carbonyl group produced fragment ions containing an A or a B ring. The fragment ions containing an A ring were common product ions of seven compounds in both ion modes, and fragment ions containing the B ring were used to judge the different substituent groups at the 3′-position. The fragmentation patterns of seven structurally related C-glycosyl quinochalcones were analyzed systematically and the formation of the fragment ions in two modes is explained in detail in this report. UPLC/Q-TOFMS is an effective tool for characterizing a complex sample, which gives higher resolution separation and generates accurate mass measurement of the product ions. Copyright ? 2008 John Wiley & Sons, Ltd.