文献类型：期刊文献

英文题名：Ultrasensitive and selective assay of glutathione species in arsenic trioxide-treated leukemia HL-60 cell line by molecularly imprinted polymer decorated electrochemical sensors

作者：Zhang, Bo[1];Liu, Jie[1];Ma, Xiaoru[1];Zuo, Peng[3];Ye, Bang-ce[2,3];Li, Yingchun[1,2]

机构：[1]Shihezi Univ, Sch Pharm, Minist Educ, Key Lab Xinjiang Phytomed Resources, Shihezi 832002, Peoples R China;[2]Shihezi Univ, Sch Chem & Chem Engn, Key Lab Green Proc Chem Engn Xinjiang Bingtuan, Shihezi 832003, Peoples R China;[3]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China

年份：2016

卷号：80

起止页码：491

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20160801962036);WOS:【SCI-EXPANDED(收录号:WOS:000372558500070)】；

基金：The project financially supported by the Major Program for Science and Technology Development of Shihezi University (gxjs2014-zdgg04), National Natural Science Foundation of China (81260487, 81460543, 81460566, and 21575089), the Scientific Research Foundation for the Returned Overseas Chinese Scholars from Ministry of Human Resources and Social Security of China (RSLX201301), the Pairing Program of Shihezi University with Eminent Scholar in Elite University (SDJDZ201501, and SDJDZ201502), and Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Molecularly imprinted polymer; Electrochemical sensor; Arsenic trioxide; Trace measurement; Glutathione species

摘要：Herein a pair of molecularly imprinted polymer (MIP) modified electrochemical sensors were reported to detect glutathione (GSH) and glutathione disulfide (GSSG) in arsenic trioxide-treated HL-60 cells. MIP film was in situ synthesized onto electrode surface via electro-polymerization in a facile way. The characteristics of the obtained sensors were investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Both GSH-MIP and GSSG-MIP sensors exhibit the relatively wide linear detection range and low detection limit of 1.33 x 10(-10) M (S/N =3). It is found that N-acetylcysteine and DL-homocysteine, the precursors of GSH, show little influence on the detection of glutathione species, nor did the reactants of arsenite and GSH. Such strategies were successfully applied to discriminate GSH and GSSG in cell samples with acceptable recoveries of 92.0-109.1%, and the results are comparable with classic o-phthalaldehyde fluorospectrophotometry. Moreover, the presented sensors allow for easy disclosure of the reversion of malignant phenotype in leukemia cells via glutathione species analysis. (C) 2016 Elsevier B.V. All rights reserved.