文献类型：期刊文献

中文题名：固定化酵母细胞生物合成谷胱甘肽的研究

英文题名：Study on GSH Biosynthesis by the Immobilized Yeast Cells

作者：赵旭东[1];魏东芝[1];俞俊棠[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,生物化学研究所上海200237

年份：2000

卷号：26

期号：1

起止页码：29

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：2000075251596);Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：酵母;谷胱甘肽;生物合成;腺苷

外文关键词：yeast; glutathione; biosynthesis; adenosine; regeneration of ATP

摘要：利用酵母细胞自身ＧＳＨ 合成酶和糖酵解途径再生的ＡＴＰ能够合成ＧＳＨ，在含葡萄糖０．７ｍ ｏｌ／Ｌ，硫酸镁１０ｍ ｍ ｏｌ／Ｌ，０．３ｍ ｏｌ／Ｌ磷酸钾缓冲液（ｐＨ７．０）和谷氨酸、半胱氨酸、甘氨酸各２０ｍ ｍ ｏｌ／Ｌ的１０ｍ Ｌ反应液中，加入１０ｇ（湿重）固定化酵母细胞，３０℃振荡反应８ｈ 可产生０．９１ｇ／Ｌ的ＧＳＨ。加入腺苷会降低ＧＳＨ的产量；当腺苷开始转化生成ＡＴＰ时加入前体，可明显提高ＧＳＨ的产量。实验结果初步表明：ＡＤＰ抑制ＧＳＨ的合成，ＡＴＰ的快速有效再生是ＧＳＨ 高效合成的必要条件。
Immobilized yeast cells were explored to synthesize GSH by utilizing both cellular GSH synthetase and ATP regeneration system in the presence of glucose. Under optimal conditions, about 9.1g/L GSH was produced by 10g immobilized yeast cells in 10mL reaction solution containing 0.7mol/L glucose, 10mmol/L MgSO 4, 0.3mol/L pH7.0 potassium phosphate buffer, 20mmol/L glutamate, 20mmol/L cysteine and 20mmol/L glycine after shaking at 30℃ for 8h. To increase ATP concentration, adenosine was added to reaction system which was converted to ATP by glycolytic pathway, adenosine kinase and adenylate kinase. However, GSH productivity decreased as 5mol/L adenosine added. On the other hand, GSH production was improved much when a glycolytic intermediate FDP was added at the beginning of ATP formation from adenosine. The experimental results shew that ADP inhibits GSH biosynthesis and it is necessary for high yield of GSH to regenerate ATP by a rapid and efficient approach.