文献类型：期刊文献

英文题名：Exploration and optimization of conditions for quantitative analysis of lignans in Schisandra chinensis by an online supercritical fluid extraction with supercritical fluid chromatography system

作者：Dai, Zhuoshun[1];Xin, Huaxia[1];Fu, Qing[1];Hao, Hongyuan[2];Li, Qiang[2];Liu, Qiaoxia[2];Jin, Yu[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China;[2]Shimadzu China Co LTD, Shanghai Branch, Analyt Applicat Ctr, Analyt Instruments Dept, Shanghai, Peoples R China

年份：2019

卷号：42

期号：14

起止页码：2444

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20192707123879);WOS:【SCI-EXPANDED(收录号:WOS:000481853700013)】；

基金：This work was supported by SHIMADZU (China) Co., Ltd.

语种：英文

外文关键词：quantitative analysis; Schisandra chinensis; supercritical fluid chromatography; supercritical fluid extraction

摘要：An online supercritical fluid extraction with supercritical fluid chromatography system could provide sequential extraction and quantitative analysis of lignans in Schisandra chinensis. Supercritical fluid extraction conditions were optimized at 15 MPa, 50 degrees C, and 4 min with supercritical CO2 adding 1% methanol; the elution volume and flow rate were set at 6 mL and 2 mL/min to blow extract out of the tank completely. The split-flow rate was confirmed at 2.5%, which determines injection volume and accuracy of quantitative detection. The factors having negative influences on supercritical fluid chromatography retention in the online system, including sample loading forms and backpressure settings, are discussed in the paper. At last, an extraction-quantitative method for lignans in Schisandra chinensis was developed, which could be finished within 19.5 min. The total content percentage of four lignans (Schisandrin, Schisandrin A, Schisandrin B and Schisandrol B) in four batches was respectively measured to be 1.42, 1.54, 1.62, and 1.90%.