文献类型：期刊文献

中文题名：流场特性对鸟苷发酵过程放大的影响

英文题名：Effect of Flow Field Characteristics on Guanosine Fermentation Process Scale-up

作者：陈双喜[1];夏建业[2];郑明英[3]

机构：[1]河南大学生命科学学院生物工程研究所,河南开封475004;[2]华东理工大学生物反应器国家重点实验室,上海200237;[3]广东肇庆星湖生物科技股份有限公司,广东肇庆526060

年份：2013

卷号：44

期号：2

起止页码：146

中文期刊名：中国医药工业杂志

外文期刊名：Chinese Journal of Pharmaceuticals

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：河南省教育厅科技攻关项目(2008A180003)

语种：中文

中文关键词：鸟苷;流场;发酵;放大

外文关键词：guanosine; flow field; fermentation; scale-up

摘要：采用计算流体力学方法,模拟了12 m3中试罐和100 m3生产罐的流场特性,确认溶氧(DO)是鸟苷发酵过程放大的瓶颈。与生产罐相比,中试罐流场较均匀,对气体控制力强,平均最大氧传递速率为65 mmol.L-1.h-1,是生产罐的15倍,能够保证发酵过程中DO维持约20%,放罐时鸟苷产率为34.02 g/L。而生产罐中由于流场特性不佳,16～32 h存在DO跌零的现象,放罐时鸟苷产率仅为20.35 g/L。
Flow field characteristics of 12 m3 middle test fermentor and 100 m3 industrial fermentor were simulated by computational fluid dynamics method. Dissolved oxygen （DO） was confirmed as the bottleneck of guanosine fermentation process scale-up. Compared with industrial fermentor, flow field of middle test fermentor was more uniform with higher control on air. The average maximum of oxygen transfer rate was 65 mmol.L^-1.h^-1, 15 times higher than that of industrial fermentor. In middle test fermentor, DO was about 20%, the final guanosine yield was 34.02 g/L. In industrial fermentor, with worse flow field characteristics, DO was about zero at 16 - 32 h, and the final guanosine yield was only 20.35 g/L.