文献类型：期刊文献

英文题名：Methanogenic degradation of branched alkanes in enrichment cultures of production water from a high-temperature petroleum reservoir

作者：Chen, Jing[1,2];Liu, Yi-Fan[1,2];Zhou, Lei[1,2];Mbadinga, Serge Maurice[1,2];Yang, Tao[1,2];Zhou, Jing[1,2];Liu, Jin-Feng[1,2];Yang, Shi-Zhong[1,2];Gu, Ji-Dong[3];Mu, Bo-Zhong[1,2,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Univ Hong Kong, Sch Biol Sci, Pokfulam Rd, Hong Kong, Peoples R China;[4]East China Univ Sci & Technol, Engn Res Ctr Microbial Enhanced Oil Recovery, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2019

卷号：103

期号：5

起止页码：2391

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20190206363180);WOS:【SCI-EXPANDED(收录号:WOS:000461392700031)】；

基金：This work was supported by the National Nature Science Foundation of China (Grants No. 41530318, 41373070), NSFC/RGC Joint Research Fund (No. 41161160560), Shanghai Fundamental Research Program (15JC1401400), and the Fundamental Research Funds for the Central Universities (No. 222201817017).

语种：英文

外文关键词：Alkylsuccinate; iso-Alkanes or branched alkanes; High-temperature petroleum reservoir; Methanogenic degradation; assA gene

摘要：Branched alkanes are important constituents of crude oil and are usually regarded as resistant to microbial degradation, resulting in little knowledge of biochemical processes involved in anaerobic branched alkanes biodegradation. Here, we initiated an incubation study by amendment of iso-C9 (2-methyl, 3-methyl, and 4-methyloctane) as substrates for methanogenic degradation in production water from a high-temperature petroleum reservoir. Over an incubation period of 367days, significant methanogenesis was observed in samples amended with these branched alkanes. The strong methanogenic activity only observed in iso-C9 amendments suggested the presence of microbial transformation from iso-alkanes into methane. GC-MS-based examination of the original production water identified an intermediate tentatively to be iso-C9-like alkylsuccinate, but was not detected in the enrichment cultures, combined with the successful amplification of assA functional gene in inoculating samples, revealing the ability of anaerobic biodegradation of iso-C9 via fumarate addition pathway. Microorganisms affiliated with members of the Firmicutes, Synergistetes, and methanogens of genus Methanothermobacter spp. were highly enriched in samples amended with iso-C9. The co-occurrence of known syntrophic acetate oxidizers Thermoacetogenium spp. and Methanothermobacter spp. (known hydrogenotrophic methanogens) indicates a potential syntrophic acetate oxidation associated with the methanogenic biodegradation of iso-C9. These results provide some useful information on the potential biodegradation of branched alkanes via methanogenesis and also suggest that branched alkanes are likely activated via fumarate addition in high-temperature petroleum reservoirs.