文献类型：期刊文献

英文题名：Enzyme-free electrochemical sensor based on ZIF-67 for the detection of superoxide anion radical released from SK-BR-3 cells

作者：Zhang, Huanhuan[1];Cai, Xuan[1];Zhao, Hongli[1];Sun, Wenqian[1];Wang, Zhenxing[1];Lan, Minbo[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2019

卷号：855

外文期刊名：JOURNAL OF ELECTROANALYTICAL CHEMISTRY

收录：;EI(收录号：20194907788556);WOS:【SCI-EXPANDED(收录号:WOS:000505643500043)】；

基金：This research was financially supported by Natural Science Foundation of Shanghai (19ZR1412000) and the Fundamental Research Funds for the Central Universities (No. 50321101917022).

语种：英文

外文关键词：Superoxide anion; ZIF-67; Enzyme-free electrochemical sensor; Living cells

摘要：Developing nanomaterials as alternatives of enzymes is of great significance to realize qualification and quantitation of superoxide anion (O-2(center dot-)), which has been proved to play a crucial role in physiological condition. In this work, a novel electrochemical catalyst of cobalt nanoparticle doped porous cubic carbon (Co-KC) was prepared through the calcination of zeolitic imidazolate framework-67 (ZIF-67), which was obtained from one-step synthesis. Then Co-PCC was used to construct enzyme-free electrochemical sensor for the detection of O-2(center dot-). The response of H2O2, though much lower than that of O-2(center dot-), was also caught by the Co-PCC/SPCE. The fabricated sensor exhibited obvious response towards O-2(center dot-) in the range of 0.8-76.8 mu M (R-2 = 0.997) with a high sensitivity of 3.59 x 10(-4) mA mu M-1 cm(-2) and a limit of detection of 0.41 NM (S/N = 3). In addition, the proposed sensor can be used to detect O-2(center dot-) released from SK-BR-3 cells, which makes it possible to monitor the dynamic change of O-2(center dot-) in living cells. (C) 2018 Elsevier B.V. All rights reserved.