文献类型：期刊文献

英文题名：Characterization of the facultative anaerobic Pseudomonas stutzeri strain HK13 to achieve efficient nitrate and nitrite removal

作者：Ding, Lei[1];Han, Bing[1];Zhou, Jianmin[1]

机构：[1]East China Univ Sci & Technol, Natl Engn Res Ctr Ind Wastewater Detoxicat & Resou, Shanghai 200237, Peoples R China

年份：2022

卷号：118

起止页码：236

外文期刊名：PROCESS BIOCHEMISTRY

收录：;EI(收录号：20221912101715);WOS:【SCI-EXPANDED(收录号:WOS:000803859600005)】；

基金：Acknowledgments This work was supported by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (grant number: B100-C-1503) , and Shanghai Leading Academic Discipline Project (grant number: B506) .

语种：英文

外文关键词：Denitrification; Pseudomonas stutzeri HK13; Alkalophilic; Nitrate loading; Nitrite removal

摘要：A new facultative anaerobic denitrifier was isolated from anoxic activated sludge and identified as Pseudomonas stutzeri strain HK13. Single-factor experiments indicated that the optimal conditions for nitrate removal were a C/N ratio of 8, an initial pH of 7-10, a shaking speed of 100 rpm, and a temperature of 30-40 C. When 100 mg/L NO3--N and NO2--N were used separately as sole nitrogen sources, the strain exhibited efficient denitrification ability with total nitrogen (TN) removal efficiencies of 98.22% and 98.58%, respectively, in 15 h with the corresponding maximum removal rates of 20.03 and 21.77 mg/L/h. And there was no nitrite accumulation finally in the nitrate denitrification system. In addition, 97.42% total oxidized nitrogen (TON) was removed at 20 C and 93.46% NO3--Nwas reduced at 45 C, suggesting that strain HK13 had a wide range of temperature and pH to adapt to the environment. Moreover, strain HK13 exhibited a notable ability to tolerate high nitrate loading (initial NO3--N > 450 mg/L) with a TON removal amount of over 200 mg/L/day. These findings demonstrate that strain HK13 is effective for nitrogen removal in wastewaters containing high concentrations of nitrate and nitrite.